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Authordc.contributor.authorGorman Goffreri, Texia 
Authordc.contributor.authorAballay Delgado, Jimena Margarita es_CL
Authordc.contributor.authorFredes Martínez, Fernando es_CL
Authordc.contributor.authorSilva Sandoval, Marco Antonio es_CL
Authordc.contributor.authorAguillón Gutiérrez, Juan Carlos es_CL
Authordc.contributor.authorAlcaíno Contador, Héctor A. es_CL
Cita de ítemdc.identifier.citationInternational Journal for Parasitology, 27:1 (1429) 1997en_US
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractCrude and partially purified somatic (S) and excretorysecretory (ES) antigens of Fasciola hepatica were subjected to Western blot analysis in order to identify polypeptides that would enable specitic and sensitive immunodiagnosis of horse and pig fasciolosis to be undertaken. Sera from 20 horses and 20 pigs with natural infections of F. hepatica and the same number of uninfected hosts of each species were tested, together with sera from 2 pigs with Cysticercus cellufosae infections. Using crude S antigens, sera from infected horses and pigs reacted specifically with a wide range of polypeptides of 14-19, 22-30, 35-37 and 42KDa. Likewise, specific reactivity between polypeptides of 14-17, 22-30 and 40 - 42 kDa in crude ES antigens and sera from infected horses and pigs was obtained. Against the criteria of high sensitivity and speciticity, the 22-30-kDa polypeptides would appear to be the most suitable candidate antigens for use in the immunodiagnosis of fasciolosis in horses and pigs. 0 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.en_US
Patrocinadordc.description.sponsorshipFONDECYT (Pr. No. 194 - 0245).en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Títulodc.titleImmunodiagnosis of Fasciolosis in horses and pigs using Western blotsen_US
Document typedc.typeArtículo de revistaen_US

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile