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Authordc.contributor.authorTapia Pinto, Verónica 
Authordc.contributor.authorGabler Neale, Fernando es_CL
Authordc.contributor.authorMuñoz, Marcela es_CL
Authordc.contributor.authorYazigi, Roberto es_CL
Authordc.contributor.authorSelman Abuchaibe, Alberto es_CL
Authordc.contributor.authorVega Blanco, María Margarita es_CL
Authordc.contributor.authorParedes Vargas, Alfonso es_CL
Authordc.contributor.authorRomero Osses, Carmen es_CL
Admission datedc.date.accessioned2011-12-22T14:58:59Z
Available datedc.date.available2011-12-22T14:58:59Z
Publication datedc.date.issued2011
Cita de ítemdc.identifier.citationGynecologic Oncology 121 (2011) 13–23es_CL
Identifierdc.identifier.otherdoi:10.1016/j.ygyno.2010.12.341
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/124240
General notedc.descriptionArtículo de publicación ISIes_CL
Abstractdc.description.abstractObjectives. To evaluate the role of trkA receptor as a potential tumor marker in serous epithelial ovarian cancer and its relationship with the angiogenic factors expression as vascular endothelial growth factor (VEGF) and nerve growth factor (NGF). Additionally, to examine whether NGF and VEGF secreted by epithelial ovarian cancer (EOC) explants and from epithelial ovarian cancer cell line (A2780) are involved in the process of angiogenesis, such as cellular proliferation, migration and differentiation of the human endothelial cell line (EA.hy926). Methods. The mRNA levels of VEGF, NGF and trkA receptors were measured using PCR in 60 ovarian samples. Cellular localization and semi-quantitative estimation of VEGF, NGF, total trkA and p-trkA was performed using IHC in epithelial cells. NGF, total trkA and p-trkA protein were also evaluated in endothelial cells from the same tissues. Human endothelial cell line EA.hy926 was cultured with conditioned media obtained from both EOC explants and from the A2780 cell line, with or without NGF stimulus. Results. Significantly higher levels of NGF, total trkA and p-trkA protein expressions were observed in epithelial and endothelial cells in poorly differentiated EOC versus normal ovary. Interestingly, the p-trkA receptor expression level showed the most significant difference and its presence was only found in borderline tumor and EOC samples indicating the importance of trkA receptor in EOC as a potential tumor marker. A significant increase in proliferation, migration and differentiation of EA.hy926 cells was observed with NGF, and this effect was significantly reverted when NGF was immuno-blocked and when a trkA inhibitor was used, showing that NGF is an important angiogenic factor in EOC by activating its trkA receptor. Conclusion. These results indicate that p-trkA may be considered as a new potential tumor marker in EOC, and that NGF may also act as a direct angiogenic factor in EOC.es_CL
Patrocinadordc.description.sponsorshipFondo Nacional de Desarrollo Científico y Tecnológico Grants 1071036.es_CL
Lenguagedc.language.isoenes_CL
Publisherdc.publisherElsevieres_CL
Keywordsdc.subjectEpithelial ovarian canceres_CL
Títulodc.titleTyrosine kinase A receptor (trkA): A potential marker in epithelial ovarian canceres_CL
Document typedc.typeArtículo de revistaes_CL


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