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Authordc.contributor.authorSandoval, Gabriela 
Authordc.contributor.authorAndrews Farrow, Bárbara es_CL
Authordc.contributor.authorAsenjo de Leuze, Juan es_CL
Admission datedc.date.accessioned2014-01-07T13:57:26Z
Available datedc.date.available2014-01-07T13:57:26Z
Publication datedc.date.issued2012-08-21
Cita de ítemdc.identifier.citationJ. Mol. Recognit. 2012; 25: 571–579en_US
Identifierdc.identifier.otherDOI: 10.1002/jmr.2223
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/125997
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractDifferentmathematicalmodels with different degrees of complexity have been proposed to model affinity chromatography. In this work, in particular, a general rate model has been studied that considers axial dispersion, external film mass transfer, intraparticle diffusion, and kinetic effects investigating the influence in the simulations of two different relationships between the properties of the mobile phase and the affinity of different proteins to the ligand bound to the matrix. Two systems were used: Blue Sepharose and Protein A. With Blue Sepharose, an increasing linear salt gradient was used, and with Protein A, a decreasing semi-linear pH gradient. The kinetic parameters obtained in each of the two elution (adsorption/desorption) relationships studied (a power law type and an exponential type) led to very good agreements between experimental and simulated elution curves of mixtures of proteins finding that for more symmetrical peaks, the preferred elution relationship should be the exponential one, in contrast to the more asymmetrical peaks which shapes are better simulated by the power law relationship.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherJohn Wiley & Sonsen_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectaffinity chromatography; rate model; elution (adsorption/desorption) relationshipsen_US
Títulodc.titleElution relationships to model affinity chromatography using a general rate modelen_US
Document typedc.typeArtículo de revista


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile