Chaxamycins A-D, Bioactive Ansamycins from a Hyper-arid Desert Streptomyces sp.
Author
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Rateb, Mostafa E.
Author
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Houssen, Wael E.
es_CL
Author
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Arnold, Markus
es_CL
Author
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Abdelrahman, Mostafa H.
es_CL
Author
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Deng, Hai
es_CL
Author
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Harrison, William T. A.
es_CL
Author
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Okoro, Chinyere K.
es_CL
Author
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Asenjo de Leuze, Juan
es_CL
Author
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Andrews Farrow, Bárbara
es_CL
Author
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Ferguson, Gail
es_CL
Author
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Bull, Alan T.
es_CL
Author
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Goodfellow, Michael
es_CL
Author
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Ebel, Rainer
es_CL
Author
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Jaspars, Marcel
es_CL
Admission date
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2014-01-07T14:19:47Z
Available date
dc.date.available
2014-01-07T14:19:47Z
Publication date
dc.date.issued
2011-06
Cita de ítem
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JOURNAL OF NATURAL PRODUCTS Volume: 74 Issue: 6 Pages: 1491-1499 Published: JUN 2011
en_US
Identifier
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DOI: 10.1021/np200320u
Identifier
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https://repositorio.uchile.cl/handle/2250/126000
General note
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Artículo de publicación ISI
en_US
Abstract
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Streptomyces sp. strain C34, isolated from soil collected
in the Chilean hyper-arid Atacama Desert, was cultured on
different media, resulting in the isolation and identification of four
new ansamycin-type polyketides. The organism was selected for
chemical investigation on the basis of a genome-mining PCR-based
experiment targeting the gene encoding rifamycin-specific 3-amino-
5-hydroxybenzoic acid synthetase (AHBA). The isolated compounds
were structurally characterized using NMR and MS techniques
and named chaxamycins A D (1 4). Compounds 1 4
were tested for their antibacterial activity against Staphylococcus
aureus ATCC 25923 and Escherichia coli ATCC 25922 and for their
ability to inhibit the intrinsic ATPase activity of the heat shock
protein 90 (Hsp90). Chaxamycin D (4), which showed a selective
antibacterial activity against S. aureus ATCC 25923, was tested further against a panel of MRSA clinical isolates. In a virtual screening
experiment, chaxamycins A D(1 4) have also been docked into the ATP-binding pocket in the N-terminal domain of the Hsp90,
and the observed interactions are discussed.
en_US
Patrocinador
dc.description.sponsorship
SULSA
MRC
G0501107
Royal Society
JP100654
Millenium Scientific Initiative
ICM-P05-001-F