Stoichiometric Model and Metabolic Flux Analysis for Leptospirillum ferrooxidans
Author
dc.contributor.author
Merino, M. P.
Author
dc.contributor.author
Andrews Farrow, Bárbara
es_CL
Author
dc.contributor.author
Asenjo de Leuze, Juan
es_CL
Admission date
dc.date.accessioned
2014-01-09T20:32:36Z
Available date
dc.date.available
2014-01-09T20:32:36Z
Publication date
dc.date.issued
2010-11-01
Cita de ítem
dc.identifier.citation
Biotechnology and Bioengineering, Vol. 107, No. 4, November 1, 2010
en_US
Identifier
dc.identifier.issn
0006-3592
Identifier
dc.identifier.other
DOI: 10.1002/bit.22851
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/126159
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
A metabolic model for Leptospirillum ferrooxidans
was developed based on the genomic information of an
analogous iron oxidizing bacteria and on the pathways of
ferrous iron oxidation, nitrogen and CO2 assimilation based
on experimental evidence for L. ferrooxidans found in the
literature. From this metabolic reconstruction, a stoichiometric
model was built, which includes 86 reactions describing
the main catabolic and anabolic aspects of its
metabolism. The model obtained has 2 degrees of freedom,
so two external fluxes were estimated to achieve a determined
and observable system. By using the external oxygen
consumption rate and the generation flux biomass as input
data, a metabolic flux map with a distribution of internal
fluxes was obtained. The results obtained were verified with
experimental data from the literature, achieving a very good
prediction of the metabolic behavior of this bacterium at
steady state.