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Authordc.contributor.authorMartínez, Patricio 
Authordc.contributor.authorGálvez, Sebastián es_CL
Authordc.contributor.authorOhtsuka, Norimasa es_CL
Authordc.contributor.authorBudinich Abarca, Marko es_CL
Authordc.contributor.authorCortés Burgos, María Paz es_CL
Authordc.contributor.authorSerpell, Cristián es_CL
Authordc.contributor.authorNakahigashi, Kenji es_CL
Authordc.contributor.authorHirayama, Akiyoshi es_CL
Authordc.contributor.authorTomita, Masaru es_CL
Authordc.contributor.authorSoga, Tomoyoshi es_CL
Authordc.contributor.authorMartínez Aguilera, Servet es_CL
Authordc.contributor.authorMaass Sepúlveda, Alejandro es_CL
Authordc.contributor.authorParada, Pilar es_CL
Admission datedc.date.accessioned2014-02-11T19:27:44Z
Available datedc.date.available2014-02-11T19:27:44Z
Publication datedc.date.issued2013
Cita de ítemdc.identifier.citationMetabolomics (2013) 9:247–257en_US
Identifierdc.identifier.otherDOI 10.1007/s11306-012-0443-3
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/126385
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractIn this study, we present the first metabolic profiles for two bioleaching bacteria using capillary electrophoresis coupled with mass spectrometry. The bacteria, Acidithiobacillus ferrooxidans strain Wenelen (DSM 16786) and Acidithiobacillus thiooxidans strain Licanantay (DSM 17318), were sampled at different growth phases and on different substrates: the former was grown with iron and sulfur, and the latter with sulfur and chalcopyrite. Metabolic profiles were scored from planktonic and sessile states. Spermidine was detected in intra- and extracellular samples for both strains, suggesting it has an important role in biofilm formation in the presence of solid substrate. The canonical pathway for spermidine synthesis seems absent as its upstream precursor, putrescine, was not present in samples. Glutathione, a catalytic activator of elemental sulfur, was identified as one of the most abundant metabolites in the intracellular space in A. thiooxidans strain Licanantay, confirming its participation in the sulfur oxidation pathway. Amino acid profiles varied according to the growth conditions and bioleaching species. Glutamic and aspartic acid were highly abundant in intra- and extracellular extracts. Both are constituents of the extracellular matrix, and have a probable role in cell detoxification. This novel metabolomic information validates previous knowledge from in silico metabolic reconstructions based on genomic sequences, and reveals important biomining functions such as biofilm formation, energy management and stress responses.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherSpringeren_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectBioleachingen_US
Títulodc.titleMetabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantayen_US
Document typedc.typeArtículo de revista


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile