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Authordc.contributor.authorRetamal Merino, Patricio 
Authordc.contributor.authorFresno, Marcela 
Authordc.contributor.authorDougnac, Catherine 
Authordc.contributor.authorGutiérrez, Sindy 
Authordc.contributor.authorGornall, Vanessa 
Authordc.contributor.authorVidal, Roberto 
Authordc.contributor.authorVernal Astudillo, Rolando 
Authordc.contributor.authorPujol, Myriam 
Authordc.contributor.authorBarreto, Marlen 
Authordc.contributor.authorGonzález Acuña, Daniel 
Authordc.contributor.authorAbalos Pineda, Pedro 
Cita de ítemdc.identifier.citationFrontiers in Microbiology. May 2015, Volume 6, Article 464en_US
Identifierdc.identifier.otherDOI: 10.3389/fmicb.2015.00464
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractSalmonella enterica serotype Enteritidis is a worldwide zoonotic agent that has been recognized as a very important food-borne bacterial pathogen, mainly associated with consumption of poultry products. The aim of this work was to determine genotypic and phenotypic evidence of S. Enteritidis transmission among seabirds, poultry and humans in Chile. Genotyping was performed using PCR-based virulotyping, pulse-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Pathogenicity-associated phenotypes were determined with survival to free radicals, acidic pH, starvation, antimicrobial resistance, and survival within human dendritic cells. As result of PCR and PFGE assays, some isolates from the three hosts showed identical genotypic patterns, and through MLST it was determined that all of them belong to sequence type 11. Phenotypic assays show diversity of bacterial responses among isolates. When results were analyzed according to bacterial host, statistical differences were identified in starvation and dendritic cells survival assays. In addition, isolates from seabirds showed the highest rates of resistance to gentamycin, tetracycline, and ampicillin. Overall, the very close genetic and phenotypic traits shown by isolates from humans, poultry, and seabirds suggest the inter-species transmission of S. Enteritidis bacteria between hosts, likely through anthropogenic environmental contamination that determines infection of seabirds with bacteria that are potentially pathogenic for other susceptible organism, including humans.en_US
Patrocinadordc.description.sponsorshipFondecyt 11110398-1070464en_US
Publisherdc.publisherFrontiers Research Foundationen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectSalmonella entericaen_US
Títulodc.titleGenetic and phenotypic evidence of the Salmonella enterica serotype Enteritidis human-animal interface in Chileen_US
Document typedc.typeArtículo de revistaen_US

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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile