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Authordc.contributor.authorFernández, N. 
Authordc.contributor.authorMontalvo, S. 
Authordc.contributor.authorFernández Polanco, F. 
Authordc.contributor.authorGuerrero, L. 
Authordc.contributor.authorCortés, I. 
Authordc.contributor.authorBorja, R. 
Authordc.contributor.authorSánchez, E. 
Authordc.contributor.authorTravieso, L. 
Cita de ítemdc.identifier.citationProcess Biochemistry 42 (2007) 721–728en_US
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractUsing the scanning electronic microscopy, it was observed that natural zeolite possesses excellent physical characteristics as a support medium in anaerobic fluidized bed reactors (AFBR). Samples for biomass analysis were taken from two identical laboratory-scale AFBR (R-1 and R-2), which were operated with 25% of fluidization. These reactors treated distillery wastewaters (vinasses) at mesophilic temperature (30 2 8C). The experiments were carried out with 0.25–0.50 and 0.50–0.80 mm zeolite particle diameter in reactors R-1 and R-2, respectively. The biomass concentration attached to zeolite in both reactors was found to be in the range of 40–45 g volatile solids/l. COD removal efficiencies as high as 90% were achieved at organic loading rate (OLRs) of up to 20 g COD/l day. The volatile fatty acid (VFA) levels were always lower that the suggested limits for digester failure. The yield coefficient of methane production was 0.29 l CH4(at STP)/g COD consumed and was virtually independent of the OLR applied. A hybridization technique (fluorescence in situ hybridization, FISH) helped determine the predominant anaerobic microorganisms that colonized the natural zeolite, which were found to be Methanosaeta and Methanosarcinaceae, observing a reduced number of sulphate reducing bacteria. The results obtained for reactors R-1 and R-2 were very similar, showing that the particle size did not significantly influence the microbial community immobilized on zeolite.en_US
Patrocinadordc.description.sponsorshipFONDECYT Project No. 1020201, Chile, and to the "Ministerio de Educación y Ciencia" and "Junta de Anadalucía" of Spainen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectAnaerobic fluidized bed reactors (AFBR)en_US
Keywordsdc.subjectScanning electronic microscopy (SEM)en_US
Keywordsdc.subjectMicrobial communitiesen_US
Keywordsdc.subjectFluorescence in situ hybridization (FISH) techniqueen_US
Títulodc.titleReal evidence about zeolite as microorganisms immobilizer in anaerobic fluidized bed reactorsen_US
Document typedc.typeArtículo de revista

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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile