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Authordc.contributor.authorFernández, Tomás 
Authordc.contributor.authorPalomino Mackenney, Jaime 
Authordc.contributor.authorParraguez Gamboa, Víctor 
Authordc.contributor.authorPeralta Troncoso, Óscar 
Authordc.contributor.authorReyes Solovera, Mónica de los 
Cita de ítemdc.identifier.citationAnimal Reproduction Science 167 (2016) 59–67en_US
Identifierdc.identifier.otherDOI: 10.1016/j.anireprosci.2016.02.005
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractGrowth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15) playimportant functions in follicular and oocyte development in many species. This study eval-uated the dynamic expression of GDF-9 and BMP-15 in canine follicles cells using flowcytometry analysis. Follicular cells were removed from three sizes of antral follicles (small,medium and large) from ovaries of bitches throughout the estrus cycle. Cells were incu-bated with anti-human GDF-9 polyclonal and anti-mouse BMP-15 monoclonal antibodies.A size and complexity discriminatory gate was used for the cytometryc analysis in theinitial dot plot and, additionally, a CD45 marker for leukocyte and propidium iodide (PI)were used for erythrocyte and debris discrimination. The evidence corroborated the pres-ence of both proteins in canine follicle cells, but these proteins were not expressed equallyduring follicular development. The results analyzed by ANOVA showed that GDF-9 expres-sion decreased (P < 0.05) during follicular growth in anestrus and proestrous/estrous, butincreased in diestrus (P < 0.05). The expression levels of BMP-15 rose (P < 0.05) from small tomedium sizes in anestrous without changing at diestrus. Small antral follicles expressed thehighest values of GDF-9 at anestrus while only BMP-15 showed higher value in small antralfollicles at proestrous-estrus compared to diestrus and anestrus. Both proteins decreasedin proestrous/estrous (P < 0.05) with increasing follicle size, registering the lowest levels inlarge follicles. The flow cytometric assay was able to assess GDF-9 and BMP-15 expressionin canine follicular cells, showing that these proteins were differentially expressed duringfollicular development, possibly related to the special features of canine reproduction.en_US
Patrocinadordc.description.sponsorshipNational Commission for Scientific and Technological Research, Chile (CONICYT) 1140658 EQM120156en_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectAntral folliclesen_US
Keywordsdc.subjectGrowth factorsen_US
Keywordsdc.subjectEstrus cycleaen_US
Títulodc.titleDifferential expression of GDF-9 and BMP- 15 duringfollicular development in canine ovaries evaluated by flowcytometryen_US
Document typedc.typeArtículo de revistaen_US
Indexationuchile.indexArtículo de publicación SCOPUS

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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile