Large Differences in Bacterial Community Composition among Three Nearby Extreme Waterbodies of the High Andean Plateau
Author
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Aguilar, Pablo
Author
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Scota, Eduardo
Author
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Dorador, Cristina
Author
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Sommaruga, Rubén
Admission date
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2016-11-24T18:50:33Z
Available date
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2016-11-24T18:50:33Z
Publication date
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2016
Cita de ítem
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Frontiers in Microbiology June 2016 Volume 7 Article 976
es_ES
Identifier
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10.3389/fmich.2015.00976
Identifier
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https://repositorio.uchile.cl/handle/2250/141432
Abstract
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The high Andean plateau or Altiplano contains different waterbodies that are subjected to extreme fluctuations in abiotic conditions on a daily and an annual scale. The bacterial diversity and community composition of those shallow waterbodies is largely unexplored, particularly, of the ponds embedded within the peatland landscape (i.e., Bofedales). Here we compare the small-scale spatial variability <1 m) in bacterial diversity and community composition between two of those ponds with contrasting apparent color, using 454 pyrosequencing of the 16S rRNA gene. Further, we compared the results with the nearest (80 m) main lagoon in the system to elucidate the importance of different environmental factors such as salinity and the importance of these ponds as a source of shared diversity. Bacterial diversity was higher in both ponds than in the lagoon and community composition was largely different among them and characterized by very low operational taxonomic unit sharing. Whereas the "green" pond with relatively low dissolved organic carbon (DOC) concentration (33.5 mg L-1) was dominated by Proteobacteria and Bacteroidetes, the one with extreme DOC concentration (424.1 mg L-1) and red hue was dominated by Cyanobacteria. By contrast, the lagoon was largely dominated by Proteobacteria, particularly by Gammaproteobacteria. A large percentage (47%) of all reads was unclassified suggesting the existence of large undiscovered bacterial diversity. Our results suggest that even at the very smallscale spatial range considered, local environmental factors are important in explaining differences in bacterial community composition in those systems. Further, our study highlights that Altiplano peatland ponds represent a hitherto unknown source of microbial diversity
es_ES
Patrocinador
dc.description.sponsorship
National Commission for Scientific and Technological Research (FONDECYT) 1140179
National Commission for Scientific and Technological Research (CONICYT, Chile) 1110953
Austrian Science Fund (FWF) F24442-B25