Improvement in carbohydrate and phlorotannin extraction from Macrocystis pyrifera using carbohydrate active enzyme from marine Alternaria sp as pretreatment

Abstract

The commercial importance of brown seaweed has been increasing over the past decade, especially due to industries interested in the extraction of phycocolloids and, more recently, of polyphenol compounds such as phlorotannins. The objective of this work was to optimize the extraction conditions of carbohydrates and phlorotannins from Macrocystis pyrifera, evaluated enzymatic pretreatment and different parameters of extraction using design of experiment. The optimal conditions upon extraction of the carbohydrates and phlorotannins were determined by means of a pretreatment protocol taking advantage on a carbohydrate active enzyme, followed by an alkaline hydrolysis with 0.5 N NaOH at 100 degrees C, 180 min, and S/L ratio of 1/20. In order to extract the carbohydrates, the best conditions found for the pretreatment procedure were 37 degrees C, pH 7.0 for 24 h, and a S/L ratio of 1/10, giving an extraction yield (EY) of 89.67 +/- 12.3 wt.%. In turn, for the extraction of phlorotannins, the best conditions identified in terms of the pretreatment were 25 degrees C, pH 7.0 for 36 h, and a S/L ratio of 1/20, thus giving a yield (EY) of 2.14 +/- 0.25 wt.%. Statistical analysis of both processes revealed a maximum EY of 91.24 wt.% for carbohydrates and 3.31 wt.% EY for phlorotannins.