Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering
Author
dc.contributor.author
Galarza, Janeth I.
Author
dc.contributor.author
Gimpel, Javier A.
Author
dc.contributor.author
Rojas, Verónica
Author
dc.contributor.author
Arredondo Vega, Bertha O.
Author
dc.contributor.author
Henríquez, Vitalia
Admission date
dc.date.accessioned
2018-07-20T17:18:59Z
Available date
dc.date.available
2018-07-20T17:18:59Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Algal Research, 31 (2018): 291–297
es_ES
Identifier
dc.identifier.other
10.1016/j.algal.2018.02.024
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/150095
Abstract
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The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.