Myogenic differentiation potential of mesenchymal stem cells derived from fetal bovine bone marrow
Author
dc.contributor.author
Okamuraa, Lucas Hidenori
Author
dc.contributor.author
Cordero, Paloma
Author
dc.contributor.author
Palomino Mackenney, Jaime
Author
dc.contributor.author
Parraguez Gamboa, Víctor
Author
dc.contributor.author
Torres, Cristian Gabriel
Author
dc.contributor.author
Peralta Troncoso, Óscar
Admission date
dc.date.accessioned
2018-07-26T14:56:47Z
Available date
dc.date.available
2018-07-26T14:56:47Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Animal Biotechnology, 29: 1, 1-11
es_ES
Identifier
dc.identifier.other
10.1080/10495398.2016.1276926
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/150300
Abstract
dc.description.abstract
The myogenic potential of bovine fetal MSC (bfMSC) derived from bone marrow (BM) remains unknown; despite its potential application for the study of myogenesis and its implications for livestock production. In the present study, three protocols for in vitro myogenic differentiation of bfMSC based on the use of DNA methyltransferase inhibitor 5-Aza-2-deoxycytidine (5-Aza), myoblast-secreted factor Galectin-1 (Gal-1), and myoblast culture medium SkGM-2 BulletKit were used. Plastic-adherent bfMSC were isolated from fetal BM collected from abattoir-derived fetuses. Post-thaw viability analyses detected 85.6% bfMSC negative for propidium iodine (PI). Levels of muscle regulatory factors (MRF) MYF5, MYF6, MYOD, and DES mRNA were higher (P<0.05) in bfMSC cultured under 100 mu M of 5-Aza compared to 1 and 10 mu M. Treatment of bfMSC with 10 mu M of 5-Aza resulted in down-regulation of MYOD mRNA (Days 7 to 21) and up-regulation of MYF6 (Day 7), MYF5, and DES mRNA (Day 21). Gal-1 and SkGM-2 BulletKit induced sequential down-regulation of early MRF (MYF5) and up-regulation of intermediate (MYOD) and late MRF (DES) mRNA. Moreover, DES and MYF5 were immunodetected in differentiated bfMSC. In conclusion, protocols evaluated in bfMSC induced progress into myogenic differentiation until certain extent evidenced by changes in MRF gene expression.
es_ES
Patrocinador
dc.description.sponsorship
Office of Research and Development, University of Chile
2014-69975
grant Fondequip from the National Commission for Scientific and Technology Research (Conicyt) from the Ministry of Education, Government of Chile
EQM120156