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Authordc.contributor.authorPokrant Huerta, Ekaterina Valerievna 
Authordc.contributor.authorMaddaleno Toledo, Aldo 
Authordc.contributor.authorAraya Ascuy, Carolina Andrea 
Authordc.contributor.authorSan Martín Núñez, Betty 
Authordc.contributor.authorCornejo, Javiera 
Admission datedc.date.accessioned2018-08-01T16:49:49Z
Available datedc.date.available2018-08-01T16:49:49Z
Publication datedc.date.issued2018
Cita de ítemdc.identifier.citationJournal of the Brazilian Chemical Society Volumen: 29 Número: 3 Páginas: 659-668es_ES
Identifierdc.identifier.other10.21577/0103-5053.20170180
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/150540
Abstractdc.description.abstractFor the detection of tetraciclines in feathers, muscle and liver, an internal protocol was designed for in-house validation of two methods, through high performance liquid chromatography with tandem mass spectrometric (HPLC-MS/MS) techniques. This protocol was based on the recommendation of the decision 2002/657/EC from the European Community as well as the food and drug administration (FDA) VICHGL49. Limit of detection (LOD) was set at 20 mu g kg(-1) and the limit of quantification (LOQ) ranged from 21.5 to 24.2, 21.2 to 21.6, and 25.0 to 27.7 mu g kg(-1) in feahers, muscle and liver samples, respectively, for all analytes. The calibrations curves show a coefficient of determination (R-2) above 0.98, 0.99 and 0.96 for feathers, muscle and liver samples, respectively. Analyte recovery ranged from 92 to 108%. In conclusion, these methods can be deemed accurate and reliable, and their validation is a fundamental step to be performed in depletion studies on these matrices.es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherSoc Brasileira Químicaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceJournal of the Brazilian Chemical Societyes_ES
Keywordsdc.subjectIn-house validationes_ES
Keywordsdc.subjectHPLC-MS/MSes_ES
Keywordsdc.subjectTetracyclineses_ES
Keywordsdc.subjectFeatherses_ES
Keywordsdc.subjectBroiler chickenses_ES
Títulodc.titleIn-House validation of HPLC-MS/MS methods for detection and quantification of tetracyclines in edible tissues and feathers of broiler chickenses_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorrgfes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile