Author | dc.contributor.author | Reyes Solovera, Mónica de los | |
Author | dc.contributor.author | Almendra, C. | |
Author | dc.contributor.author | Berland, M. | |
Author | dc.contributor.author | Del Campo, H. | |
Author | dc.contributor.author | Barros, C. | |
Admission date | dc.date.accessioned | 2018-12-20T14:52:34Z | |
Available date | dc.date.available | 2018-12-20T14:52:34Z | |
Publication date | dc.date.issued | 1996 | |
Cita de ítem | dc.identifier.citation | Archivos de Medicina Veterinaria, Volumen 28, Issue 1, 2018, Pages 31-38 | |
Identifier | dc.identifier.issn | 0301732X | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/157123 | |
Abstract | dc.description.abstract | The swim-up and Percoll gradient techniques were evaluated for the selection of frozen bovine spermatozoa to be used on an in vitro fertilization system. The parameters assayed were: concentration, morphology, motility and rate of in vitro fertilization and rate of development. The spermatozoa were from a single bull and the oocytes were aspirated from ovaries obtained at the slaughter house and matured in vitro. Sperm motility increased significantly (p<0.05) from 70%-75% to 90% - 95%. However after sperm selection there was no significant difference between both methods (p>0.05). Sperm concentration after swim-up was 3.33 × 106 sp/ml and with Percoll 16.5 × 106 sp/ml (p<0.01). Samples obtained by both methods showed a decrease in abnormal spermatozoa as compared to controls (17.3% in swim-up; 24.8% in Percoll and 47% in control) (p<0.01). There was a significant difference between both methods only in regards to anomalies of the neck region (3.5% in swim-up and 9.6% in Percoll (p>0.0 | |
Lenguage | dc.language.iso | en | |
Source | dc.source | Archivos de Medicina Veterinaria | |
Keywords | dc.subject | In vitro fertilization | |
Keywords | dc.subject | Percoll | |
Keywords | dc.subject | Spermatozoa | |
Keywords | dc.subject | Swim-up | |
Título | dc.title | Selection of frozen bull spermatozoa for in vitro fertilization Selección de espermatozoides de toro para fecundación in vitro | |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso a solo metadatos | |
Cataloguer | uchile.catalogador | apc | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |