Author | dc.contributor.author | Abalos Pineda, Pedro | |
Author | dc.contributor.author | Daffner S., José | |
Author | dc.contributor.author | Pinochet Valenzuela, Lautaro | |
Admission date | dc.date.accessioned | 2018-12-20T14:52:35Z | |
Available date | dc.date.available | 2018-12-20T14:52:35Z | |
Publication date | dc.date.issued | 2000 | |
Cita de ítem | dc.identifier.citation | Veterinary Microbiology, Volumen 71, Issue 1-2, 2000, Pages 161-167 | |
Identifier | dc.identifier.issn | 03781135 | |
Identifier | dc.identifier.other | 10.1016/S0378-1135(99)00141-8 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/157131 | |
Abstract | dc.description.abstract | An O-polysaccharide (O-chain) and a hot-water extracted polysaccharide (PS), both obtained from Brucella abortus 1119-3, and a B. melitensis 16M native hapten (NH) were evaluated by indirect enzyme linked immunosorbent assay (ELISA) on three groups of cattle sera. The sera tested were: (a) 75 sera from cows naturally infected with B. abortus; (b) 130 sera from non-infected and non-vaccinated cattle; and © 61 sera from non-infected heifers recently vaccinated with B. abortus Strain 19 (S19). Sensitivity (Se), specificity (Sp) and the capability to discriminate vaccinated cattle (ADV) were determined.Using PS antigen, Se was 100% and the Sp was 97.7%, while the highest Sp was obtained by using the O-chain (99.2%). For the NH antigen, Se was 94.7% and the Sp was 90.0%. The ADV of the three antigens was approximately 85%.Statistical analysis showed significant differences between O-chain/PS and O-chain/NH antigens. The agreement among antigens determined by kappa coefficient was 0.899 for O-chain/PS, 0.845 for O-chain/NH and 0.795 for PS/NH. | |
Lenguage | dc.language.iso | en | |
Source | dc.source | Veterinary Microbiology | |
Keywords | dc.subject | Brucella sp. | |
Keywords | dc.subject | Cattle | |
Keywords | dc.subject | ELISA | |
Keywords | dc.subject | S19 vaccine | |
Keywords | dc.subject | Soluble antigens | |
Título | dc.title | Evaluation of three Brucella soluble antigens used in an indirect Elisa to discriminate S19 vaccinated from naturally infected cattle | |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso a solo metadatos | |
Cataloguer | uchile.catalogador | apc | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |