| Author | dc.contributor.author | Aguillón Gutiérrez, Juan Carlos | |
| Author | dc.contributor.author | Smith, P. | |
| Author | dc.contributor.author | Colombo, A. | |
| Author | dc.contributor.author | Molina, M. C. | |
| Author | dc.contributor.author | Ferreira, A. | |
| Admission date | dc.date.accessioned | 2018-12-20T14:53:10Z | |
| Available date | dc.date.available | 2018-12-20T14:53:10Z | |
| Publication date | dc.date.issued | 1997 | |
| Cita de ítem | dc.identifier.citation | Arch. med. vet. v.29 n.1 Valdivia 1997 | |
| Identifier | dc.identifier.issn | 0301732X | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/157257 | |
| Abstract | dc.description.abstract | Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Igs. This antibody was affinity purified in a Sepharose-protein G column. By immunoradiometric assay against the immunogen, the rabbit IgG anti-salmon Igs showed a high specific titer (at least 1/100.000) for the antigen. This reagent offers the possibility of detection of salmon or trout (crossreactivity) humoral immune responses, both for diagnostic and research purposes. | |
| Lenguage | dc.language.iso | sp | |
| Source | dc.source | Archivos de Medicina Veterinaria | |
| Keywords | dc.subject | Diagnosis | |
| Keywords | dc.subject | Polyclonal antibodies | |
| Keywords | dc.subject | Salmon | |
| Título | dc.title | Anticuerpos policlonales antiinmunoglobulinas
de salmón; herramientas potenciales para diagnóstico | |
| Title in another language | dc.title.alternative | Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis | |
| Document type | dc.type | Artículo de revista | |
| dcterms.accessRights | dcterms.accessRights | Acceso a solo metadatos | |
| Cataloguer | uchile.catalogador | laj | |
| Indexation | uchile.index | Artículo de publicación SCOPUS | |
| uchile.cosecha | uchile.cosecha | SI | |
