Hunting for cultivable Micromonospora strains in soils of the Atacama Desert
Author
dc.contributor.author
Carro, Lorena
Author
dc.contributor.author
Razmilic, Valeria
Author
dc.contributor.author
Nouioui, Imen
Author
dc.contributor.author
Richardson, Lee
Author
dc.contributor.author
Pan, Che
Author
dc.contributor.author
Golinska, Patrycja
Author
dc.contributor.author
Asenjo, Juan
Author
dc.contributor.author
Bull, Alan
Author
dc.contributor.author
Klenk, Hans
Author
dc.contributor.author
Goodfellow, Michael
Admission date
dc.date.accessioned
2019-05-31T15:19:11Z
Available date
dc.date.available
2019-05-31T15:19:11Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, Volumen 111, Issue 8, 2018, Pages 1375-1387
Identifier
dc.identifier.issn
15729699
Identifier
dc.identifier.issn
00036072
Identifier
dc.identifier.other
10.1007/s10482-018-1049-1
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/169344
Abstract
dc.description.abstract
Innovative procedures were used to selectively isolate small numbers of Micromonospora
strains from extreme hyper-arid and high altitude
Atacama Desert soils. Micromonosporae were recognised on isolation plates by their ability to produce
filamentous microcolonies that were strongly
attached to the agar. Most of the isolates formed
characteristic orange colonies that lacked aerial
hyphae and turned black on spore formation, whereas
those from the high altitude soil were dry, blue-green
and covered by white aerial hyphae. The isolates
were assigned to seven multi- and eleven singlemembered groups based on BOX-PCR profiles.
Representatives of the groups were assigned to either
multi-membered clades that also contained marker strains or formed distinct phyletic lines in the
Micromonospora 16S rRNA gene tree; many of the
isolates were considered to be putatively novel
species of Micromonospora. Most of the isolates
from the high altitude soils showed activity against
wild type strains of Bacillus subtilis and Pseudomonas fluorescens while those from the
rhizosphere of Parastrephia quadrangulares and
from the Lomas Bayas hyper-arid soil showed
resistance to UV radiation.