Modifying internal organization and surface morphology of siRNA lipoplexes by sodium alginate addition for efficient siRNA delivery
Author
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Arruda, Danielle Campiol
Author
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Gonzalez, Ismael José
Author
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Finet, Stéphanie
Author
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Cordova, Luis
Author
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Trichet, Valérie
Author
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Andrade, Gracielle Ferreira
Author
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Hoffmann, Céline
Author
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Bigey, Pascal
Author
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de Almeida Macedo, Waldemar Augusto
Author
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Da Silva Cunha, Armando
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Malachias de Souza, Angelo
Author
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Escriou, Virgi
Admission date
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2019-10-15T12:23:30Z
Available date
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2019-10-15T12:23:30Z
Publication date
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2019
Cita de ítem
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Journal of Colloid and Interface Science, Volumen 540,
Identifier
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10957103
Identifier
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00219797
Identifier
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10.1016/j.jcis.2019.01.043
Identifier
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https://repositorio.uchile.cl/handle/2250/171562
Abstract
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Vectorized small interfering RNAs (siRNAs) are widely used to induce specific mRNA degradation in the intracellular compartment of eukaryotic cells. Recently, we developed efficient cationic lipid-based siRNA vectors (siRNA lipoplexes or siLex) containing sodium alginate (Nalg-siLex) with superior efficiency and stability properties than siLex. In this study, we assessed the physicochemical and some biological properties of Nalg-siLex compared to siLex. While no significant differences in size, ζ potential and siRNA compaction were detected, the addition of sodium alginate modified the particle morphology, producing smoother and heterogeneous particles characterized by transmission electron microscopy. We also noted that Nalg-siLex have surface differences observed by X-ray photoelectron spectroscopy. These differences could arise from an internal reorganization of components induced by the addition of sodium alginate, that is indicated by Small-Angle X-ray Scatteri