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Authordc.contributor.authorWilkens, Camila A. 
Authordc.contributor.authorVishwanathan, Nandita 
Authordc.contributor.authorBaltes, Nicholas J. 
Authordc.contributor.authorLucero, Alicia T. 
Authordc.contributor.authorHu, Wei Shou 
Authordc.contributor.authorGerdtzen, Ziomara P. 
Admission datedc.date.accessioned2019-10-15T12:23:57Z
Available datedc.date.available2019-10-15T12:23:57Z
Publication datedc.date.issued2019
Cita de ítemdc.identifier.citationJournal of Chemical Technology and Biotechnology, Volumen 94, Issue 5, 2019, Pages 1488-1498
Identifierdc.identifier.issn10974660
Identifierdc.identifier.issn02682575
Identifierdc.identifier.other10.1002/jctb.5906
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/171657
Abstractdc.description.abstractBACKGROUND: Reducing lactate production in animal cell cultures has been reported to improve cell culture performance and productivity of recombinant protein. A novel genome editing tool, CRISPR/Cas, has been used widely to induce double-strand breaks in the genome and introduce targeted mutations efficiently. RESULTS: In the present work, we used a publicly available human-codon optimized CRISPR/Cas system to introduce mutations in one of the LDHa gene copies to obtain an LDHa single-allele knockout Chinese hamster ovary (CHO) cell clone to analyze its effect over cell metabolism. Fed-batch cultures were conducted in order to evaluate the culture performance of mutant cells. Results show that cell growth was reduced and metabolism was modified by the LDHa single-allele knockout, whereas specific protein rate and volumetric production were greatly enhanced. Additionally, the first in-depth analysis of the metabolic effects of LDHa single allele knock
Lenguagedc.language.isoen
Publisherdc.publisherJohn Wiley and Sons Ltd
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Chemical Technology and Biotechnology
Keywordsdc.subjectCHO cells
Keywordsdc.subjectCRISPR/Cas
Keywordsdc.subjectlactate dehydrogenase a (LDHa)
Keywordsdc.subjectmammalian cell engineering
Keywordsdc.subjectmetabolic flux analysis
Títulodc.titleAn LDHa single allele CHO cell mutant exhibits altered metabolic state and enhanced culture performance
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile