Sleep loss disrupts morning-to-evening differences in human white adipose tissue transcriptome

Abstract

Context: Chronodisruption, as caused by such conditions as perturbations of 24-hour rhythms of physiology and behavior, may promote the development of metabolic diseases. Objective: To assess the acute effects of sleep curtailment on circadian regulation (i.e., morning-to-evening differences) of white adipose tissue (WAT) transcriptome in normal-weight men. Design: Fifteen healthy men aged 18 to 30 years (mean 6 SEM, 24.0 ± 0.9years) were studied. In randomized, balanced order they underwent three separate nights with regular sleep duration (8 hours of sleep between 11:00 PM and 7:00 AM), sleep restriction (4 hours of sleep between 3:00 AM and 7:00 AM), and sleep deprivation (no sleep at all). Sleep was polysomnographically evaluated. WAT biopsy samples were taken twice at 9:00 PM and 7:00 AM to assess morning-to-evening differences. WAT transcriptome profile was assessed by RNA sequencing, and expression of relevant circadian core clock genes were an