Surfactant-mediated enzymatic superactivity in water/ionic liquid mixtures, evaluated on a model hydrolytic reaction catalyzed by α-chymotrypsin
Author
dc.contributor.author
Calderón, C.
Author
dc.contributor.author
Contreras, R.
Author
dc.contributor.author
Campodónico, R.
Admission date
dc.date.accessioned
2019-10-22T03:13:49Z
Available date
dc.date.available
2019-10-22T03:13:49Z
Publication date
dc.date.issued
2019
Cita de ítem
dc.identifier.citation
Journal of Molecular Liquids, Volumen 283,
Identifier
dc.identifier.issn
01677322
Identifier
dc.identifier.other
10.1016/j.molliq.2019.03.106
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/171990
Abstract
dc.description.abstract
In this work, the influence of the C 12 -, C 14 -, and C 16 -alkyl chain derivatives of 1-alkyl-3-methyl-imidazole tetrafluoroborate over the hydrolisis of p-nitrophenyl trimethylacetate catalyzed by α-chymotrypsin was studied in water and water - ionic liquids mixtures. The ionic liquid used is 1-butyl-3-methyl-imidazole tetrafluoroborate (BMIMBF 4 ). 1-alkyl-3-methylimidazole tetrafluoroborate derived surfactants can increase the catalytic efficiency of α-chymotrypsin on the hydrolysis of p-nitrophenyl trimethylacetate. This effect is negatively affected by the decrease on the surfactant's critical micelle concentration (CMC) and the partition of the substrate between the micelles and the external media observed above the critical miscellar concentration (CMC). In water/BMIMBF 4 mixtures, the presence of the ionic liquid (IL), render the C 14 and C 16 surfactants insoluble, and overrides the effect elicited by the C 12 alkyl chain surfactant on the activity of α-chymotrypsin. The loss of the surfactants influence on the enzymatic activity due to the presence of BMIMBF 4 seems to be a complex process, controlled by a decreased affinity between α-chymotrypsin and the surfactant molecules and, to a larger extent, the appearance of an “acquired resistance” of the enzyme to the influence of the surfactants, related to a more compact and resilient conformation of the protein in the presence of BMIMBF 4 . The influence of BMIMBF 4 is not only limited to the surfactant/enzyme interaction, but also it does modifies the partition of the substrate between the aqueous media and the micellar environment.