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Authordc.contributor.authorCahuascanco, Berly 
Authordc.contributor.authorBahamonde, Javiera 
Authordc.contributor.authorHuaman, Olger 
Authordc.contributor.authorJervis, Miguel 
Authordc.contributor.authorCortez, Jahaira 
Authordc.contributor.authorPalomino, Jaime 
Authordc.contributor.authorEscobar, Alejandro 
Authordc.contributor.authorRetamal Merino, Patricio 
Authordc.contributor.authorTorres, Cristian G. 
Authordc.contributor.authorPeralta, Óscar Alejandro 
Admission datedc.date.accessioned2019-10-22T03:14:59Z
Available datedc.date.available2019-10-22T03:14:59Z
Publication datedc.date.issued2019
Cita de ítemdc.identifier.citationVeterinary Research, Volumen 50, Issue 1, 2019
Identifierdc.identifier.issn12979716
Identifierdc.identifier.issn09284249
Identifierdc.identifier.other10.1186/s13567-019-0643-1
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/172050
Abstractdc.description.abstractStaphylococcus aureus is the most commonly isolated pathogen from clinical bovine mastitis samples and a difficult pathogen to combat. Mesenchymal stem cells (MSC) are multipotent progenitor cells equipped with a variety of factors that inhibit bacterial growth. The aim of the present study was to evaluate the in vitro antibacterial potential against S. aureus of conditioned medium (CM) from MSC derived from fetal bovine bone marrow (BM-MSC) and adipose tissue (AT-MSC). BM-MSC, AT-MSC and fetal fibroblasts (FB) cultures were activated by infection with S. aureus. Bacterial growth was evaluated in presence of CM, concentrated CM (CCM), activated CM (ACM) and concentrated ACM (CACM) from BM-MSC, AT-MSC and FB. Gene expression of β-defensin 4A (bBD-4A), NK-lysine 1 (NK1), cathelicidin 2 (CATHL2), hepcidin (HEP) and indoleamine 2,3 dioxygenase (IDO) and protein expression of bBD-4A were determined in activated and non-activated cells. The majority of BM-MSC and AT-MSC expressed CD73, Oct4 and Nanog, and were negative for CD34. Growth of S. aureus decreased when it was exposed to CM from BM-MSC, AT-MSC and FB. Moreover, growth of S. aureus in CCM, ACM and CACM was lower compared to controls of CM from BM-MSC and AT-MSC. Activated AT-MSC increased mRNA levels of bBD4A and NK1, and protein levels of bBD4A in CM. Thus, CM from fetal bovine BM-MSC and AT-MSC has the capacity to reduce in average ~30% of S. aureus relative growth under in vitro conditions. The in vitro antibacterial effect of fetal bovine MSC may be mediated by bBD4A and NK1 activity.
Lenguagedc.language.isoen
Publisherdc.publisherBioMed Central
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceVeterinary Research
Keywordsdc.subjectVeterinary (all)
Títulodc.titleBovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureus
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorlaj
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile