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Authordc.contributor.authorPeña Villalobos, Isaac 
Authordc.contributor.authorCasanova Maldonado, Ignacio J. 
Authordc.contributor.authorLois, Pablo 
Authordc.contributor.authorSabat Kirkwood, Alejandro Pablo 
Authordc.contributor.authorPalma, Verónica 
Admission datedc.date.accessioned2019-10-30T15:40:05Z
Available datedc.date.available2019-10-30T15:40:05Z
Publication datedc.date.issued2019
Cita de ítemdc.identifier.citationFrontiers in Physiology, Volumen 10, Issue JAN, 2019,
Identifierdc.identifier.issn1664042X
Identifierdc.identifier.other10.3389/fphys.2018.01821
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/172531
Abstractdc.description.abstractCopyright © 2019 Peña-Villalobos, Casanova-Maldonado, Lois, Sabat and Palma.Several studies have evaluated plastic changes in the morphology of the digestive tract in rodents subjected to caloric restriction or restricted availability. Nevertheless, studies that link these morphological responses to physiological consequences are scarce. In order to investigate short-term plastic responses in the intestine, we acclimated adult Mus musculus (BALB/c) males for 20 days to four distinctive treatments: two caloric regimens (ad libitum and 60% of calorie ingestion) and two levels of periodicity of the regimens (continuous and stochastic treatment). At the end of the treatment we analyzed the cell proliferation and cell death dynamics of small intestinal crypts in these animals. In addition, we measured organ masses and lengths, hydrolytic digestive enzyme activities, and energy output from feces. Finally, in order to explore the metabolic changes generated by these dietary conditions we assessed the catabolic activity (i.e., enzymes) of the liver. Our results show that individuals acclimated to a continuous and 60% regimen presented longer intestines in comparison to the other treatments. Indeed, their intestines grew with a rate of 0.22 cm/day, generating a significant caloric reduction in the content of their feces. Besides, both mass and intestinal lengths were predicted strongly by the stabilization coefficient of BrdU+ proliferating cells per crypt, the latter correlating positively with the activity of n-aminopeptidases. Interestingly, by using pharmacological inhibition of the kinase mammalian target of rapamycin complex 1 (mTORC1) by Rapamycin, we were able to recapitulate similar changes in the proliferation dynamics of intestinal stem cells. Based on our results, we propose that the impact of caloric restriction on macroscopic variation in morphology and functional changes in digestive n-aminopeptidases occurs through synchronization in the proliferation rate of stem and/or progenitor cells located in the small intestinal crypts and requires mTORC1 as a key mediator. Hence, we suggest that an excessive stem and progenitor activity could result in increased crypts branching and might therefore underlie the reported intestinal tissue expansion in response to short-term caloric restriction. Summarizing, we demonstrate for the first time that short-term caloric restriction induces changes in the level of cell proliferation dynamics explaining in part digestive tract plasticity in adaptive performance.
Lenguagedc.language.isoen
Publisherdc.publisherFrontiers Media S.A.
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceFrontiers in Physiology
Keywordsdc.subjectCaloric restriction
Keywordsdc.subjectEnvironmental stochasticity
Keywordsdc.subjectIntestinal crypts
Keywordsdc.subjectJejunum
Keywordsdc.subjectMTORC1
Keywordsdc.subjectStarvation
Títulodc.titleAdaptive physiological and morphological adjustments mediated by intestinal stem cells in response to food availability in mice
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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