TRPV1-Estradiol Stereospecific Relationship Underlies Cell Survival in Oxidative Cell Death
Author
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Ramírez Barrantes, Ricardo
Author
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Carvajal Zamorano, Karina
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Rodríguez, Belén
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Córdova, Claudio
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Lozano, Carlo
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Simón, Felipe
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Díaz, Paula
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Muñoz, Pablo
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Marchant, Ivanny
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Latorre, Ramón
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Castillo, Karen
Author
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Olivero, Pablo
Admission date
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2020-08-04T00:12:59Z
Available date
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2020-08-04T00:12:59Z
Publication date
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2020
Cita de ítem
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Frontiers in Physiology May 2020 | Volume 11 | Article 444
es_ES
Identifier
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10.3389/fphys.2020.00444
Identifier
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https://repositorio.uchile.cl/handle/2250/176275
Abstract
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17 beta-estradiol is a neuronal survival factor against oxidative stress that triggers its protective effect even in the absence of classical estrogen receptors. The polymodal transient receptor potential vanilloid subtype 1 (TRPV1) channel has been proposed as a steroid receptor implied in tissue protection against oxidative damage. We show here that TRPV1 is sufficient condition for 17 beta-estradiol to enhance metabolic performance in injured cells. Specifically, in TRPV1 expressing cells, the application of 17 beta-estradiol within the first 3 h avoided H2O2-dependent mitochondrial depolarization and the activation of caspase 3/7 protecting against the irreversible damage triggered by H2O2. Furthermore, 17 beta-estradiol potentiates TRPV1 single channel activity associated with an increased open probability. This effect was not observed after the application of 17 alpha-estradiol. We explored the TRPV1-Estrogen relationship also in primary culture of hippocampal-derived neurons and observed that 17 beta-estradiol cell protection against H2O2-induced damage was independent of estrogen receptors pathway activation, membrane started and stereospecific. These results support the role of TRPV1 as a 17 beta-estradiol-activated ionotropic membrane receptor coupling with mitochondrial function and cell survival.
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Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
Fondecyt 11100047
DIUV 21
ACT-1104
Fondecyt 1121078
FONDECYT 1180999
FONDECYT 1190203
Iniciativa Cientifica Milenio of the Ministry of Economy, Development and Tourism (Chile)
Millennium Scientific Initiative of the Chilean Ministry of Economy, Development, and Tourism
P029-022-F