Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process
Author
dc.contributor.author
González, Carol
Author
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Abarca González, Daniela Alejandra
Author
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Zúñiga, Rommy N.
Author
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Estay, Humberto
Author
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Troncoso, Elizabeth
Admission date
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2020-10-28T21:33:15Z
Available date
dc.date.available
2020-10-28T21:33:15Z
Publication date
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2020
Cita de ítem
dc.identifier.citation
Foods 2020, 9, 913
es_ES
Identifier
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10.3390/foods9070913
Identifier
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https://repositorio.uchile.cl/handle/2250/177431
Abstract
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This work deepens our understanding of starch digestion and the consequent absorption
of hydrolytic products generated in the human small intestine. Gelatinized starch dispersions were
digested with -amylase in an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane
process. This study innovates with respect to the existing literature, because it considers the impact
of simultaneous digestion and absorption processes occurring during the intestinal digestion of
starchy foods and adopts phenomenological models that deal in a more realistic manner with the
behavior found in the small intestine. Operating the i-IDS at di erent flow/dialysate flow ratios
resulted in distinct generation and transfer curves of reducing sugars mass. This indicates that the
operating conditions a ected the mass transfer by di usion and convection. However, the transfer
process was also a ected by membrane fouling, a dynamic phenomenon that occurred in the i-IDS.
The experimental results were extrapolated to the human small intestine, where the times reached
to transfer the hydrolytic products ranged between 30 and 64 min, according to the flow ratio used.
We consider that the i-IDS is a versatile system that can be used for assessing and/or comparing
digestion and absorption behaviors of di erent starch-based food matrices as found in the human
small intestine, but the formation and interpretation of membrane fouling requires further studies for
a better understanding at physiological level. In addition, further studies with the i-IDS are required
if food matrices based on fat, proteins or more complex carbohydrates are of interest for testing.
Moreover, a next improvement step of the i-IDS must include the simulation of some physiological
events (e.g., electrolytes addition, enzyme activities, bile, dilution and pH) occurring in the human
small intestine, in order to improve the comparison with in vivo data.
es_ES
Patrocinador
dc.description.sponsorship
National Commission for Scientific and Technological Research
(CONICYT Chile) through FONDECYT project 11140543, FONDECYT project 1191858, and project fund
CONICYT-PIA Project AFB180004.