Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells
Author
dc.contributor.author
Rajivgandhi, Govindan
Author
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Saravanan, Kandasamy
Author
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Ramachandran, Govindan
Author
dc.contributor.author
Li, Jia-Ling
Author
dc.contributor.author
Yin, Lingzi
Author
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Quero, Franck
Author
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Alharbi, Naiyf S.
Author
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Kadaikunnan, Shine
Author
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Khaled, Jamal M.
Author
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Manoharan, Natesan
Author
dc.contributor.author
Li, Wen-Jun
Admission date
dc.date.accessioned
2021-05-31T20:03:09Z
Available date
dc.date.available
2021-05-31T20:03:09Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
International Journal of Biological Macromolecules 164 (2020) 4010–4021
es_ES
Identifier
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10.1016/j.ijbiomac.2020.08.169
Identifier
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https://repositorio.uchile.cl/handle/2250/179893
Abstract
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In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, beta-morindone, alpha-copaene, 9-H-pyrido[3,4-b]indole, beta-thujene and terpinolene. The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential. The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface. The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 mu g/ml(-1). Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis. The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity. Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
es_ES
Patrocinador
dc.description.sponsorship
National Natural Science Foundation of China (NSFC)
41950410573
91951205
31670009
China Postdoctoral Science Foundation
2019M663213
King Saud University
RSP-2020/70