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Authordc.contributor.authorBorie Polanco, Consuelo 
Authordc.contributor.authorBravo Vásquez, Cristian 
Authordc.contributor.authorDettleff Faúndes, Phillip 
Authordc.contributor.authorGalarce Gálvez, Nicolás 
Authordc.contributor.authorDorner Ampuero, Jessica 
Authordc.contributor.authorMartínez Moncada, Víctor 
Cita de ítemdc.identifier.citationElectronic Journal of Biotechnology 49 (2021) 1-4es_ES
Abstractdc.description.abstractBackground: Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence genes with other B. canis strains. Results: Genome assembly produced a total length of 3,289,216 bp, N50 of 95,163 and GC% of 57.27, organized in 54 contigs in chromosome I, and 21 contigs in chromosome II. The genome annotation identified a total of 1981 CDS, 3 rRNA and 36 tRNA in chromosome I, and 1113 CDS and 10 tRNA in chromosome II. There is little variation between the different strains and the SCL isolate. Phylogenetic analysis showed that the Chilean SCL strain is closely related to B. canis and B. suis strains. Small differences were found when compared to the Serbian isolate, but all strains shared the same recent common ancestor. Finally, changes in the sequence of some virulence factors showed that the SCL strain is similar to other South American B. canis strains. Conclusions: This work sequenced and characterized the complete genome of B. canis strain SCL, evidencing the complete presence of all the genes of the virB operon, and minor changes in outer membrane proteins and in the urease operon. Our data suggest that B. canis was introduced from North America and then spread throughout the South American continent. How to cite: Borie C, Bravo C, Dettleff P, et al. First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins.es_ES
Patrocinadordc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1180544es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceElectronic Journal of Biotechnologyes_ES
Keywordsdc.subjectBrucella canises_ES
Keywordsdc.subjectCanine brucellosises_ES
Keywordsdc.subjectSCL straines_ES
Keywordsdc.subjectUrease operones_ES
Keywordsdc.subjectvirB operones_ES
Keywordsdc.subjectVirulence geneses_ES
Títulodc.titleFirst genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical originses_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile