Multi-trait GWAS using imputed high-density genotypes from whole-genome sequencing identifies genes associated with body traits in Nile tilapia
Yoshida, Grazyella M.
Yáñez López, José
Cita de ítem
BMC Genomics (2021) 22:57
Background: Body traits are generally controlled by several genes in vertebrates (i.e. polygenes), which in turn
make them difficult to identify through association mapping. Increasing the power of association studies by
combining approaches such as genotype imputation and multi-trait analysis improves the ability to detect
quantitative trait loci associated with polygenic traits, such as body traits.
Results: A multi-trait genome-wide association study (mtGWAS) was performed to identify quantitative trait loci
(QTL) and genes associated with body traits in Nile tilapia (Oreochromis niloticus) using genotypes imputed to
whole-genome sequences (WGS). To increase the statistical power of mtGWAS for the detection of genetic
associations, summary statistics from single-trait genome-wide association studies (stGWAS) for eight different body
traits recorded in 1309 animals were used. The mtGWAS increased the statistical power from the original sample
size from 13 to 44%, depending on the trait analyzed. The better resolution of the WGS data, combined with the
increased power of the mtGWAS approach, allowed the detection of significant markers which were not previously
found in the stGWAS. Some of the lead single nucleotide polymorphisms (SNPs) were found within important
functional candidate genes previously associated with growth-related traits in other terrestrial species. For instance,
we identified SNP within the α1,6-fucosyltransferase (FUT8), solute carrier family 4 member 2 (SLC4A2), A disintegrin
and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) and heart development protein with EGF like domains
1 (HEG1) genes, which have been associated with average daily gain in sheep, osteopetrosis in cattle, chest size in
goats, and growth and meat quality in sheep, respectively.
Conclusions: The high-resolution mtGWAS presented here allowed the identification of significant SNPs, linked to
strong functional candidate genes, associated with body traits in Nile tilapia. These results provide further insights
about the genetic variants and genes underlying body trait variation in cichlid fish with high accuracy and strong
Production Development Corporation (CORFO project) a Chilean governmental organization 14EIAT-28667
Fondecyt/Conicyt Postdoctoral Grant 3190553
Nucleo Milenio INVASAL - Chile's government program, Iniciativa Cientifica Milenio from Ministerio de Economia, Fomento y Turismo
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