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Authordc.contributor.authorForno Bell, Natalia
Authordc.contributor.authorMuñoz, Marcos A.
Authordc.contributor.authorChacón, Óscar
Authordc.contributor.authorPachá, Paulina
Authordc.contributor.authorIragüen, Daniela
Authordc.contributor.authorCornejo Kelly, Javiera
Authordc.contributor.authorSan Martín Núñez, Betty Verónica
Cita de ítemdc.identifier.citationFrontiers in Veterinary Science March 2021 | Volume 8 | Article 572568es_ES
Abstractdc.description.abstractSynergy or additive effect between Aloe vera (L.) Burm.f. and beta-lactam (b-lactam) antibiotics has been reported against Staphylococcus aureus, one of the most important etiological agents of cow mastitis. The goal of the present study was to predict the efficacy of intramammary formulations containing the Aloe vera gel extract in the combination with cloxacillin or ceftiofur at low concentrations in lactating cows as an alternative therapy. Each quarter of 20 healthy Holstein Friesian lactating cows were treated with a single dose of one of the following formulations, corresponding to one of these treatment groups: A1, A2, A3, and A4. A1 and A2 contained cloxacillin at 0.25 and 0.5 mg/ml, whereas A3 and A4 contained ceftiofur 0.25 and 0.5 mg/ml, respectively. In addition, all formulations contained 600 mg/ml of an alcoholic extract of Aloe vera. Milk samples were taken at predefined time points. Antibiotics and aloin (active compound of Aloe vera) concentrations were assessed by liquid chromatography mass spectrometry system (LC-MS/MS). Pharmacokinetic profiles were obtained, and the efficacy index, the fraction of dosing interval in which the antimicrobial concentration remains above the minimum inhibitory concentrations (MICs) (T > MIC) for each formulation, was calculated considering MIC values against Staphylococcus aureus ATCC 29213 as obtained for the combination Aloe vera+antibiotic and aloin concentration in the extract.Mammary gland safety assessment was performed for each combination. Values of the main efficacy index for this study, T > MIC (h) for Aloe vera were 23.29, 10.50, 27.50, and 13.89, whereas for cloxacillin or ceftiofur were 19.20, 10.9, 19.74, and 15.63, for A1, A2, A3, and A4, respectively. Only A1 and A3 reached aloin and antibiotic recommended values as predictors of clinical efficacy for cloxacillin, ceftiofur, and aloin (50, 70, and 60%, respectively), assuming a dose interval of 24 h. The efficacy index values obtained suggest that A1 and A3 might be an effective therapy to treat bovine mastitis caused by S. aureus after a single dose. Nevertheless, further trials in S. aureus mastitis clinical cases are mandatory to confirm the efficacy of Aloe vera formulations.es_ES
Patrocinadordc.description.sponsorshipCONICYT (National Commission for Science and Technology from the Government of Chile) CONICYT-PCHA/doctorado Nacional/2015-21150305 FONDEF from CONICYT (National Commission for Science and Technology from the Government of Chile) IT14I10013es_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers in Veterinary Sciencees_ES
Keywordsdc.subjectCow mastitises_ES
Keywordsdc.subjectStaphyhcoccus aureuses_ES
Keywordsdc.subjectAloe vera (L.) Burm. f.es_ES
Keywordsdc.subjectIntramammary formulationses_ES
Keywordsdc.subjectEfficacy indexes_ES
Títulodc.titleEfficacy prediction of four pharmaceutical formulations for intramammary administration containing Aloe vera (L.) Burm. f. combined with ceftiofur or cloxacillin in lactating cows as an alternative therapy to treat mastitis caused by staphylococcus aureuses_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES
Indexationuchile.indexArtículo de publicación SCOPUSes_ES

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Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States