Browsing by Author "Contreras, Inés"

Now showing items 1-14 of 14

    • Analysis of Two Complementary Single-Gene Deletion Mutant Libraries of Salmonella Typhimurium in Intraperitoneal ... 
      Silva Valenzuela, Cecilia; Molina Quiroz, Roberto; Desai, Prerak; Valenzuela, Camila; Porwollik, Steffen; Zhao, Ming; Hoffman, Robert M.; Andrews-Polymenis, Helene; Contreras, Inés; Santiviago Cid, Carlos; McClelland, Michael (Frontiers Media, 2016)
      Two pools of individual single gene deletion (SOD) mutants of S. Typhimurium 14028s encompassing deletions of 3,923 annotated non-essential ORFs and sRNAs were screened by intraperitoneal (IP) injection in BALB/c mice ...
    • Different sugar residues of the lipopolysaccharide outer core are required for early interactions of ... 
      Bravo, Denisse; Hoare, Anilei; Silipo, Alba; Valenzuela, Camila; Salinas, Catalina; Álvarez Armijo, Sergio Aníbal; Molinaro, Antonio; Valvano, Miguel A.; Contreras, Inés (Elsevier, 2011)
      The role of lipopolysaccharide (LPS) in entry of Salmonella Typhimurium into epithelial cells remains unclear. In this study, we tested the ability of a series of mutants with deletions in genes for the synthesis and ...
    • Epithelial cell contact-induced alterations in Salmonella enterica serovar Typhi lipopolysaccharide are ... 
      Lyczak, J. B.; Zaldi, T. S.; Grout, M.; Bittner, M.; Contreras, Inés; Pier, G. B. (BLACKWELL SCIENCE LTD, 2001-11)
      The invasion of Pseudomonas aeruginosa and Salmonella enterica serovar Typhi into epithelial cells depends on the cystic fibrosis transmembrane conductance regulator (CFTR) protein as an epithelial receptor. In the case ...
    • Exposure to sub-inhibitory concentrations of cefotaxime enhances the systemic colonization of Salmonella ... 
      Molina Quiroz, Roberto C.; Silva, Cecilia A.; Molina, Cristian F.; Leiva, Lorenzo E.; Reyes Cerpa, Sebastián; Contreras, Inés; Santiviago Cid, Carlos (Royal Soc., 2015)
      It has been proposed that sub-inhibitory concentrations of antibiotics play a role in virulence modulation. In this study, we evaluated the ability of Salmonella enterica serovar Typhimurium (hereafter S. Typhimurium) to ...
    • Growth-phase regulation of lipopolysaccharide O-antigen chain length influences serum resistance in serovars ... 
      Bravo Rodríguez, Denisse; Silva Valenzuela, Cecilia Alejandra; Carter Jaña, Javier Antonio; Hoare Teuche, Anilei Paz; Álvarez Armijo, Sergio Aníbal; Blondel, Carlos; Zaldívar San Román, María Mercedes; Valvano, Miguel A.; Contreras, Inés (SOC GENERAL MICROBIOLOGYJournal Of Medical Microbiology, vol. 57 Issue 8 p. 938-946 AUG 2008, 2008-08)
      The amount of lipopolysaccharide (LPS) O antigen (OAg) and its chain length distribution are important factors that protect bacteria from serum complement. Salmonella enterica serovar Typhi produces LPS with long chain ...
    • Infection of Mice by Salmonella enterica Serovar Enteritidis Involves Additional Genes That Are Absent in ... 
      Silva, Cecilia A.; Blondel, Carlos J.; Quezada, Carolina P.; Porwollik, Steffen; Andrews Polymenis, Helene L.; Toro, Cecilia S.; Zaldívar San Román, María Mercedes; Contreras, Inés; McClelland, Michael; Santiviago Cid, Carlos (AMER SOC MICROBIOLOGY, 2012-02)
      Salmonella enterica serovar Enteritidis causes a systemic, typhoid-like infection in newly hatched poultry and mice. In the present study, a library of 54,000 transposon mutants of S. Enteritidis phage type 4 (PT4) strain ...
    • O-antigen expression in Salmonella enterica serovar Typhi is regulated by nitrogen availability through ... 
      Bittner, Mauricio; Saldías, Soledad; Estévez, C.; Zaldívar San Román, María Mercedes; Marolda, Cristina; Valvano, Miguel A.; Contreras, Inés (SOC GENERAL MICROBIOLOGY, 2002-12)
      The authors previously reported increased expression of the Salmonella enterica serovar Typhi (S. typhi) rfaH gene when the bacterial cells reach stationary phase. In this study, using a lacZ fusion to the rfaH promoter ...
    • O-antigen modal chain length in Shigella flexneri 2a is growth-regulated through RfaH-mediated transcriptional ... 
      Carter Jaña, Javier Antonio; Blondel, Carlos; Zaldívar San Román, María Mercedes; Álvarez Armijo, Sergio Aníbal; Marolda, Cristina L.; Valvano, Miguel A.; Contreras, Inés (SOC GENERAL MICROBIOLOGY, 2007-10)
      Shigella flexneri 2a 2457T produces lipopolysaccharide (LIPS) with two O-antigen (OAg) chain lengths: a short (S-OAg) controlled by WzzB and a very long (VL-OAg) determined by Wzz(pHS-2). This study demonstrates that the ...
    • Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is ... 
      Pezoa, David; Blondel Buijuy, Carlos José; Silva Valenzuela, Cecilia Alejandra; Yang, Hee-Jeong; Andrews Polymenis, Helene; Santiviago Cid, Carlos; Contreras, Inés (BioMed Central, 2014)
      The type VI secretion system (T6SS) is a virulence factor for many Gram-negative bacteria. Salmonella genus harbors five phylogenetically distinct T6SS loci encoded in Salmonella Pathogenicity Islands (SPIs) SPI-6, SPI-19, ...
    • The outer core lipopolysaccharide of Salmonella enterica Serovar typhi is required for bacterial entry into ... 
      Hoare, Anilei; Bittner, Mauricio; Carter, Javier; Álvarez Armijo, Sergio Aníbal; Zaldívar San Román, María Mercedes; Bravo, Denisse; Valvano, Miguel A.; Contreras, Inés (AMER SOC MICROBIOLOGY, 2006-03)
      Salmonella enterica serovar Typhi causes typhoid fever in humans. Central to the pathogenicity of serovar Typhi is its capacity to invade intestinal epithelial cells. The role of lipopolysaccharide (LPS) in the invasion ...
    • RpoS and RpoN are involved in the growth-dependent regulation of rfaH transcription and O antigen expression ... 
      Bittner, Mauricio; Saldías, Soledad; Altamirano, Francisco; Valvano, Miguel A.; Contreras, Inés (ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2004-01)
      We reported earlier that the production of 0 antigen lipopolysaccharide (LPS) by Salmonella enterica serovar Typhi (Salmonella typhi) increases at the onset of stationary phase and correlates with a growth-regulated ...
    • Spontaneous Excision of the Salmonella enterica Serovar Enteritidis-Specific Defective Prophage-Like Element SE14 
      Santiviago Cid, Carlos; Blondel, Carlos; Quezada, Carolina P.; Silva, Cecilia A.; Tobar, Pia M.; Porwollik, Steffen; McClelland, Michael; Andrews Polymenis, Helene L.; Toro, Cecilia S.; Zaldívar San Román, María Mercedes; Contreras, Inés (2010-04)
      Salmonella enterica serovar Enteritidis has emerged as a major health problem worldwide in the last few decades. DNA loci unique to S. Enteritidis can provide markers for detection of this pathogen and may reveal pathogenic ...
    • The rfaH gene, which affects lipopolysaccharide synthesis in Salmonella enterica serovar Typhi, is ... 
      Rojas, Gonzalo; Saldías, Soledad; Bittner, Mauricio; Zaldívar San Román, María Mercedes; Contreras, Inés (Elsevier Sciences B. V., 2001)
      We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi Strain Ty2. The gene shoed a high degree of similirity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A ...
    • The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic ... 
      Pezoa, David; Yang, Hee Jeong; Blondel, Carlos J.; Santiviago Cid, Carlos; Andrews Polymenis, Helene L.; Contreras, Inés (PLOS ONE, 2013)
      The role of the Salmonella Pathogenicity Islands (SPIs) in pathogenesis of Salmonella enterica Typhimurium infection in the chicken is poorly studied, while many studies have been completed in murine models. The Type VI ...