Browsing by Author "Vicuña E., Rafael"
Now showing items 1-13 of 13
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Gutiérrez Mostafá, Matías Ricardo; Rojas Soto, Luis; Mancilla Villalobos, Rodrigo; Seelenfreund Hirsch, Daniela; Vicuña E., Rafael; Lobos, S. (SPRINGER, 2008-10)In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn2+ homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been ...
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Polanco, R.; Lobos, S.; Vicuña E., Rafael (ELSEVIER SCIENCE INC, 2002-04-16)The white rot basidiomycete Ceriporiopsis subvermispora secretes the ligninolytic enzymes manganese-dependent peroxidase (MnP) and laccase to the extracellular medium. The promoter region of the laccase gene (Cs-lcsl) ...
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Vicuña E., Rafael; Cori, Osvaldo (Elsevier Science, 1981)
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Lobos, S.; Larrondo, F.; Salas, L.; Karahanian, Eduardo; Vicuña E., Rafael (ELSEVIER SCIENCE BV, 1998-01-12)A cDNA (MnP13-1) and the Cs-mnp1 gene encoding for an isoenzyme of manganese peroxidase (MnP) from C. subvermispora were isolated separately and sequenced. The cDNA, identified in a library constructed in the vector Lambda ...
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Seelenfreund Hirsch, Daniela; Vicuña E., Rafael (Stockton Press, 1990-01)The ability of the lignino-cellulolytic actinomycete Streptomycetes viridosporus T7A to attack purified fractions of kraft lignin was examined. In the presence of 0.3% yeast extract, high-molecular weight kraft lignin (MW ...
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Lobos, S.; Tello, Mario; Polanco, R.; Larrondo, L. F.; Manubens, Augusto; Salas, L.; Vicuña E., Rafael (CURRENT SCIENCE ASSN, 2001-10-25)Lignin, the most abundant renewable source of aromatic carbon on earth, consists in a highly irregular three-dimensional biopolymer of oxygenated phenylpropanoid units. In natural environments, lignin is only degraded ...
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Larrondo, L. F.; Lobos, S.; Stewart, P.; Cullen, D.; Vicuña E., Rafael (AMER SOC MICROBIOLOGY, 2001-05)We expressed cDNAs coding for manganese peroxidases (MnPs! from the basidiomycetes Ceriporiopsis subvermispora (MnP1) and Phanerochaete chrysosporium (H4) under control of the alpha -amylase promoter from. Aspergillus ...
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Rajakumar, S.; Gaskell, J.; Cullen, C.; Lobos, S.; Karahanian, Eduardo; Vicuña E., Rafael (AMER SOC MICROBIOLOGY, 1996-07)Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described ...
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Rüttimann, C.; Seelenfreund Hirsch, Daniela; Vicuña E., Rafael (Elsevier, 1987)The ability of the ligninolytic actinomycete Streptomyces viridosporus T7A to degrade selected lignin model compounds, both in the presence and in the absence of lignocellulose, was examined. Compounds studied included ...
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Goycoolea, M.; Seelenfreund Hirsch, Daniela; Rüttimann, C.; González, B.; Vicuña E., Rafael (Elsevier Inc., 1986)The lignolytic capacity of some natural bacterial isolates eas examined. Strains were selected from samples of decaying wood by growth in a minimal medium containing aromatic compounds with a structural relationship to ...
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Seelenfreund Hirsch, Daniela; Lapierre, C.; Vicuña E., Rafael (ELSEVIER SCIENCE BV, 1990-02)The actinomycete Streptomycetes viridosporus attacks wheat lignocellulose releasing soluble lignin-rich fragments (APPL). Chemical analyses indicated that these APPL contain 20% less guaiacyl and 70% less glucose residues ...
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Salas, Carlos; Lobos, S.; Larraín, Juan; Salas, Loreto; Cullen, D.; Vicuña E., Rafael (PORTLAND PRESS, 1995-06)Laccase is one of the ligninolytic enzymes found in liquid cultures of the fungus Ceriporiopsis subvermispora in defined medium. As an approach to a clarification of the role of laccases during the attack on lignin by the ...
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Karahanian, Eduardo; Corsini, Gino; Lobos, S.; Vicuña E., Rafael (ELSEVIER SCIENCE BV, 1998-11-26)A gene encoding laccase has been isolated from a genomic library of the white-rot basidiomycete Ceriporiopsis subvermispora constructed in Lambda GEM-ii. This gene (Cs-lcs1) contains an open reading frame of 2215 bp, ...