Author | dc.contributor.author | Vincent, Thierry | |
Author | dc.contributor.author | Saikali, Philippe | es_CL |
Author | dc.contributor.author | Cayrol, Romain | es_CL |
Author | dc.contributor.author | Roth Metcalfe, Alejandro Darío | es_CL |
Author | dc.contributor.author | Bar-Or, Amit | es_CL |
Author | dc.contributor.author | Prat, Alexandre | es_CL |
Author | dc.contributor.author | Antel, Jack P. | es_CL |
Admission date | dc.date.accessioned | 2010-01-26T20:04:48Z | |
Available date | dc.date.available | 2010-01-26T20:04:48Z | |
Publication date | dc.date.issued | 2008 | |
Cita de ítem | dc.identifier.citation | The Journal of Immunology, 181: 5730–5737, 2008 | en_US |
Identifier | dc.identifier.issn | 0022-1767 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/118985 | |
Abstract | dc.description.abstract | Autoantibody neuromyelitis optica-IgG (NMO-IgG) recognizing aquaporin-4 (AQP4) is implicated as playing a central role in the
physiopathology of NMO. The aim of this in vitro-based study was to characterize functional consequences of interaction between
NMO-IgG and cells of the neurovascular unit (astrocytes and brain endothelium) that would provide insight into recognized
features of NMO, namely altered blood-brain barrier (BBB) permeability and granulocyte recruitment. We used sera from NMO
and longitudinally extensive transverse myelitis cases shown to bind in a characteristic perivascular pattern to primate cerebellar
slices. Using flow cytometry, we found that sera from NMO-IgG-positive patients reacted with CNS-derived human fetal astrocytes,
whereas sera from multiple sclerosis patients did not. We demonstrated that NMO-IgG binding to astrocytes alters aquaporin-
4 polarized expression and increases permeability of a human BBB endothelium/astrocyte barrier. We further demonstrated
that NMO-IgG binding to human fetal astrocytes can result in NK cell degranulation, astrocyte killing by Ab-dependent cellular
cytotoxicity and complement-dependent granulocyte attraction through the BBB model. Our study highlights important functional
roles for NMO-IgG that could account for pathological lesions and BBB dysfunction observed in NMO. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | The American Association of Immunologists | en_US |
Keywords | dc.subject | MULTIPLE-SCLEROSIS | en_US |
Título | dc.title | Functional Consequences of Neuromyelitis Optica-IgG Astrocyte Interactions on Blood-Brain Barrier Permeability and Granulocyte Recruitment | en_US |
Document type | dc.type | Artículo de revista | |