Author | dc.contributor.author | O’Brien, Erin K. | |
Author | dc.contributor.author | d’Alencon, Claudia | es_CL |
Author | dc.contributor.author | Bonde, Gregory | es_CL |
Author | dc.contributor.author | Li, Wei | es_CL |
Author | dc.contributor.author | Schoenebeck, Jeff | es_CL |
Author | dc.contributor.author | Allende Connelly, Miguel | es_CL |
Author | dc.contributor.author | Gelb, Bruce D. | es_CL |
Author | dc.contributor.author | Yelon, Deborah | es_CL |
Author | dc.contributor.author | Eisen, Judith S. | es_CL |
Author | dc.contributor.author | Cornell, Robert A. | es_CL |
Admission date | dc.date.accessioned | 2011-04-04T12:24:48Z | |
Available date | dc.date.available | 2011-04-04T12:24:48Z | |
Publication date | dc.date.issued | 2003-09-35 | |
Cita de ítem | dc.identifier.citation | DEVELOPMENTAL BIOLOGY, Volume: 265, Issue: 1, Pages: 246-261, 2004 | en_US |
Identifier | dc.identifier.issn | 0012-1606 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119133 | |
Abstract | dc.description.abstract | The genes that control development of embryonic melanocytes are poorly defined. Although transcription factor Ap-2a is expressed in
neural crest (NC) cells, its role in development of embryonic melanocytes and other neural crest derivatives is unclear because mouse Ap-2a
mutants die before melanogenesis. We show that zebrafish embryos injected with morpholino antisense oligonucleotides complementary to
ap-2a (ap-2a MO) complete early morphogenesis normally and have neural crest cells. Expression of c-kit, which encodes the receptor for
the Steel ligand, is reduced in these embryos, and, similar to zebrafish c-kit mutant embryos, embryonic melanophores are reduced in number
and migration. The effects of ap-2a MO injected into heterozygous and homozygous c-kit mutants support the notion that Ap-2a works
through C-kit and additional target genes to mediate melanophore cell number and migration. In contrast to c-kit mutant embryos, in ap-2a
MO-injected embryos, melanophores are small and under-pigmented, and unexpectedly, analysis of mosaic embryos suggests Ap-2a
regulates melanophore differentiation through cell non-autonomous targets. In addition to melanophore phenotypes, we document reduction
of other neural crest derivatives in ap-2a MO-injected embryos, including jaw cartilage, enteric neurons, and sympathetic neurons. These
results reveal that Ap-2a regulates multiple steps of melanophore development, and is required for development of other neuronal and nonneuronal
neural crest derivatives. | en_US |
Patrocinador | dc.description.sponsorship | This work was
supported by NIH grant HD22486 to J.S.E. and a Carver
Foundation seed grant to R.A.C. C. d’., and M.A. were
supported by grants ICM P99-137-f and Fondecyt 1031003.
E.K.O. was supported by Grant T32 DC00040 (Bruce
Gantz, PI). | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
Keywords | dc.subject | Transcription factor ap-2; Zebrafish | en_US |
Título | dc.title | Transcription factor Ap-2 alpha is necessary for development of embryonic melanophores, autonomic neurons and pharyngeal skeleton in zebrafish | en_US |
Document type | dc.type | Artículo de revista | |