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Authordc.contributor.authorDorador, Cristina 
Authordc.contributor.authorVila Pinto, Irma es_CL
Authordc.contributor.authorRemonsellez, Francisco es_CL
Authordc.contributor.authorImhoff, Johannes F. es_CL
Authordc.contributor.authorWitzel, Karl-Paul es_CL
Admission datedc.date.accessioned2011-09-01T14:30:53Z
Available datedc.date.available2011-09-01T14:30:53Z
Publication datedc.date.issued2010-05-18
Cita de ítemdc.identifier.citationFEMS MICROBIOLOGY ECOLOGY, Volume: 73, Issue: 2, Pages: 291-302, 2010es_CL
Identifierdc.identifier.issn0168-6496
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/119277
General notedc.descriptionArtículo de publicación ISIes_CL
Abstractdc.description.abstractAnalyses of clone libraries from water and sediments of different sites from Salar de Huasco, a high-altitude athalassohaline wetland in the Chilean Altiplano, revealed the presence of five unique clusters of uncultured Archaea that have not been previously reported or specifically assigned. These sequences were distantly related (83–96% sequence identity) to a limited number of other clone sequences and revealed no identity to cultured Archaea. The abundance of Archaea and Bacteria was estimated using qPCR and community composition was examined through the construction of clone libraries of archaeal 16S rRNA gene. Archaea were found to be dominant over Bacteria in sediments from two saline sites (sites H4: 6.31 104 and site H6: 1.37 104 mS cm 1) and in one of the water samples (freshwater from site H0: 607 mS cm 1). Euryarchaeotal sequences were more abundant than crenarchaeotal sequences.Many of the clone sequences (52%) were similar to uncultured archaeal groups found in marine ecosystems having identity values between 99% and 97%. A major fraction of the sequences (40%) were members of Methanobacteria, while others were included in the Marine Benthic Groups B and D, the Miscellaneous Crenarchaeotic Group, the Terrestrial Miscellaneous Euryarchaeotal Group, Marine Group I and Halobacteria. The presence of uncultured archaeal groups in Salar de Huasco extends their known distribution in inland waters, providing new clues about their possible function in the environment.es_CL
Patrocinadordc.description.sponsorshipC.D. was supported by a doctoral fellowship from the German Academic Exchange Service (DAAD).es_CL
Lenguagedc.language.isoenes_CL
Publisherdc.publisherWILEY-BLACKWELL PUBLISHINGes_CL
Keywordsdc.subjectarchaeal diversityes_CL
Títulodc.titleUnique clusters ofArchaea inSalar deHuasco, an athalassohaline evaporitic basin of theChilean Altiplanoes_CL
Document typedc.typeArtículo de revista


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