Solubilization of lipid bilayers by myristyl sucrose ester: effect of cholesterol and phospholipid head group size
Author
dc.contributor.author
Toro, C.
Author
dc.contributor.author
Sánchez, S. A.
es_CL
Author
dc.contributor.author
Zanocco Loyola, Antonio
es_CL
Author
dc.contributor.author
Lemp Miranda, Else
es_CL
Author
dc.contributor.author
Gratton, E.
es_CL
Author
dc.contributor.author
Gunthera, G.
es_CL
Admission date
dc.date.accessioned
2014-01-10T13:48:07Z
Available date
dc.date.available
2014-01-10T13:48:07Z
Publication date
dc.date.issued
2009-02
Cita de ítem
dc.identifier.citation
Chemistry and Physics of Lipids 157 (2009) 104–112
en_US
Identifier
dc.identifier.other
doi:10.1016/j.chemphyslip.2008.11.004
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/121736
General note
dc.description
Artículo de publicación ISI.
en_US
Abstract
dc.description.abstract
The solubilization of biological membranes by detergents has been used as amajor method for the isolation
and purification of membrane proteins and other constituents. Considerable interest in this field has
resulted fromthe finding that differentcomponents can be solubilized selectively. Certainmembrane constituents
are incorporated into small micelles, whereas others remain in the so-called detergent-resistant
membrane domains that are large enough to be separated by centrifugation. The detergent-resistant
fractions contain an elevated percentage of cholesterol, and thus its interaction with specific lipids and
proteins may be key for membrane organization and regulation of cellular signaling events.
This report focuses on the solubilization process induced by the sucrose monoester of myristic acid,
-d-fructofuranosyl-6-O-myristyl- -d-glucopyranoside (MMS), a nonionic detergent. We studied the
effect of the head group and the cholesterol content on the process. 1-Palmitoyl-2-oleoyl-sn-glycero-
3-phosphocholine (POPC) and dioctadecyl-dimethyl-ammonium chloride (DODAC) vesicles were used,
and the solubilization processwas followed using Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene)
generalized polarization (GP) measurements, carried out in the cuvette and in the 2-photon microscope.
Our results indicate that: (i) localization of theMMSmoieties in the lipid bilayer depends on the characteristics
of the lipid polar head group and influences the solubilization process. (ii) Insertion of cholesterol
molecules into the lipid bilayer protects it from solubilizaton and (iii) the microscopic mechanism of
solubilization by MMS implies the decrease in size of the individual liposomes