Antioxidant activity of the mangiferin inclusion complex with beta-cyclodextrin
Author
dc.contributor.author
Rocha Ferreira, Fabricia da
Author
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Barros Valentim, Iara
es_CL
Author
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Catarí Ramones, Edgar Luís
es_CL
Author
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Salles Trevisan, Maria Teresa
es_CL
Author
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Olea Azar, Claudio
es_CL
Author
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Pérez Cruz, Fernanda
es_CL
Author
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Caxico de Abreu, Fabiane
es_CL
Author
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Fonseca Goulart, Marília Oliveira
es_CL
Admission date
dc.date.accessioned
2014-01-29T20:22:52Z
Available date
dc.date.available
2014-01-29T20:22:52Z
Publication date
dc.date.issued
2012-09-28
Cita de ítem
dc.identifier.citation
LWT - Food Science and Technology 51 (2013) 129e134
en_US
Identifier
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doi: 10.1016/j.lwt.2012.09.032
Identifier
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https://repositorio.uchile.cl/handle/2250/121790
General note
dc.description
Artículo de publicación ISI.
en_US
Abstract
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The aim of this study was to characterize the complex between mangiferin (MGN) with beta-cyclodextrin (beta-CD) obtained through the co-evaporation method using Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared spectroscopy (FT-IR), as well as to verify the antioxidant activity of this complex by means of radical scavenging activity toward 2,2'-diphenyl-1-picrylhydrazyl radical (RSA-DPPH.), with additional analysis of solvent effects, and Oxygen Radical Antioxidant Capacity-Fluorescein (ORAC-FL) assays. The protective effects of MGN and the MGN:beta-CD complex against peroxyl radical-initiated membrane lipid peroxidation were also evaluated. The analysis of the solvent effects in the DPPH. method demonstrated that at amounts of organic solvents lower than the ratio 50:50 (methanol-water or ethanol-water) there is a drawback, due to the hydrophobic nature of DPPH and should not be used. The antioxidant activity of the MGN:beta-CD complex was higher than in its free form, as seen by both methods. The RSA-DPPH. value was twofold larger for the complex while the ORAC-FL value was fifteenfold larger. Lipid peroxidation assays showed that the MGN:beta-CD complex has a protective effect on the membrane that is as effective as the positive control (Trolox).
en_US
Patrocinador
dc.description.sponsorship
CNPq/PROSUL, CAPES, CAPES/PNPD,
FAPEAL, BNB (Banco do Nordeste do Brasil), CNPq/PNPD (process
559277/2008-3), INCT-Bioanalítica and RENORBIO.