Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa
Author | dc.contributor.author | Cortés, Constanza J. | |
Author | dc.contributor.author | Codelia, Verónica A. | es_CL |
Author | dc.contributor.author | Manosalva, Iris | es_CL |
Author | dc.contributor.author | Lange, Johanna de | es_CL |
Author | dc.contributor.author | Reyes Solovera, Mónica de los | es_CL |
Author | dc.contributor.author | Moreno, Ricardo D. | es_CL |
Admission date | dc.date.accessioned | 2008-12-23T17:12:55Z | |
Available date | dc.date.available | 2008-12-23T17:12:55Z | |
Publication date | dc.date.issued | 2006-06 | |
Cita de ítem | dc.identifier.citation | ANIMAL REPRODUCTION SCIENCE Volume: 93 Issue: 1-2 Pages: 165-175 Published: JUN 2006 | en |
Identifier | dc.identifier.issn | 0378-4320 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/122358 | |
Abstract | dc.description.abstract | The scope of the present study was to evaluate the presence and activation of proacrosin/acrosin as a tool to determine the acrosomal status of fresh and frozen/thawed dog spermatozoa. Monoclonal antibody C5F11, directed against human acrosin, cross-reacted with dog spermatozoa and labeled the acrosome of both fresh and frozen/thawed dog spermatozoa. Frozen/thawed spermatozoa had a lesser proportion of labeled spermatozoa than fresh spermatozoa (P < 0.05). When live spermatozoa were labeled with soybean trypsin inhibitor conjugated with Alexa 488 (SBTI-Alexa 488), the proportion of acrosome-labeled fresh spermatozoa was less than frozen/thawed spermatozoa (P < 0.05). By using Western blots and enzymatic activity, frozen/thawed spermatozoa had a greater proportion of active acrosin than fresh spermatozoa. In addition, beta 1,4-galactosyl-transferase (GaIT), a plasma membrane bound protein, remained attached to frozen/thawed spermatozoa. Proacrosin is activated during freezing/thawing of dog spermatozoa, and that proacrosin/acrosin may be a good indicator of acrosomal integrity of frozen/thawed spermatozoa. | en |
Lenguage | dc.language.iso | en | en |
Publisher | dc.publisher | ELSEVIER | en |
Keywords | dc.subject | POLYSULFATE-BINDING DOMAIN | en |
Título | dc.title | Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa | en |
Document type | dc.type | Artículo de revista |
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