Polyelectrolyte complex formation mediated immobilization of chitosan-invertase neoglycoconjugate on pectin-coated chitin
Author | dc.contributor.author | Gómez, Leissy | |
Author | dc.contributor.author | Ramírez, Héctor L. | es_CL |
Author | dc.contributor.author | Neira Carrillo, Andrónico | es_CL |
Author | dc.contributor.author | Villalonga, Reynaldo | es_CL |
Admission date | dc.date.accessioned | 2009-04-07T16:50:27Z | |
Available date | dc.date.available | 2009-04-07T16:50:27Z | |
Publication date | dc.date.issued | 2006-05 | |
Cita de ítem | dc.identifier.citation | BIOPROCESS AND BIOSYSTEMS ENGINEERING Volume: 28 Issue: 6 Pages: 387-395 Published: MAY 2006 | en |
Identifier | dc.identifier.issn | 1615-7591 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/122360 | |
Abstract | dc.description.abstract | Saccharomyces cerevisiae invertase, chemically modified with chitosan, was immobilized on pectin-coated chitin support via polyelectrolyte complex formation. The yield of immobilized enzyme protein was determined as 85% and the immobilized biocatalyst retained 97% of the initial chitosan-invertase activity. The optimum temperature for invertase was increased by 10 degrees C and its thermostability was enhanced by about 10 degrees C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength Solutions and was 4-fold more resistant to thermal treatment Lit 65 degrees C than the native Counterpart. The biocatalyst prepared retained 96 and 95% of the original catalytic activity after ten cycles of reuse and 74 h of continuous operational regime in a packed bed reactor, respectively. | en |
Lenguage | dc.language.iso | en | en |
Publisher | dc.publisher | SPRINGER | en |
Keywords | dc.subject | THERMAL STABILIZATION | en |
Título | dc.title | Polyelectrolyte complex formation mediated immobilization of chitosan-invertase neoglycoconjugate on pectin-coated chitin | en |
Document type | dc.type | Artículo de revista |
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