Patterns of Piscirickettsia salmonis load in susceptible and resistant families of Salmo salar
Author
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Dettleff, Phillip
Author
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Bravo, Cristian
Author
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Patel, Alok
Author
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Martínez Moncada, Víctor
Admission date
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2015-09-04T18:24:42Z
Available date
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2015-09-04T18:24:42Z
Publication date
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2015
Cita de ítem
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Fish & Shellfish Immunology 45 (2015) 67-71
en_US
Identifier
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DOI: 10.1016/j.fsi.2015.03.039
Identifier
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https://repositorio.uchile.cl/handle/2250/133436
General note
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Artículo de publicación ISI
en_US
Abstract
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The pathogen Piscirickettsia salmonis produces a systemic aggressive infection that involves several organs and tissues in salmonids. In spite of the great economic losses caused by this pathogen in the Atlantic salmon (Selma solar) industry, very little is known about the resistance mechanisms of the host to this pathogen. In this paper, for the first time, we aimed to identify the bacterial load in head kidney and muscle of Atlantic salmon exhibiting differential familiar mortality. Furthermore, in order to assess the patterns of gene expression of immune related genes in susceptible and resistant families, a set of candidate genes was evaluated using deep sequencing of the transcriptome. The results showed that the bacterial load was significantly lower in resistant fish, when compared with the susceptible individuals. Based on the candidate genes analysis, we infer that the resistant hosts triggered up-regulation of specific genes (such as for example the LysC), which may explain a decrease in the bacterial load in head kidney, while the susceptible fish presented an exacerbated innate response, which is unable to exert an effective response against the bacteria. Interestingly, we found a higher bacterial load in muscle when compared with head kidney. We argue that this is possible due to the availability of an additional source of iron in muscle. Besides, the results show that the resistant fish could not be a likely reservoir of the bacteria.
en_US
Patrocinador
dc.description.sponsorship
Chilean Government through CONICYT
FONDECYT 1120608