Ion channels activated by light in Limulus ventral photoreceptors
Author
dc.contributor.author
Bacigalupo Vicuña, Juan
Author
dc.contributor.author
Chinn, Kevin
Author
dc.contributor.author
Lisman, John E.
Admission date
dc.date.accessioned
2018-12-20T14:41:15Z
Available date
dc.date.available
2018-12-20T14:41:15Z
Publication date
dc.date.issued
1986
Cita de ítem
dc.identifier.citation
Journal of General Physiology, Volumen 87, Issue 1, 2018, Pages 73-89
Identifier
dc.identifier.issn
15407748
Identifier
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00221295
Identifier
dc.identifier.other
10.1085/jgp.87.1.73
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/157033
Abstract
dc.description.abstract
The light-activated conductance of Limulus ventral photoreceptors was studied using the patch-clamp technique. Channels (40 pS) were observed whose probability of opening was greatly increased by light. In some cells the latency of channel activation was nearly the same as that of the macroscopic response, while in other cells the channel latency was much greater. Like the macroscopic conductance, channel activity was reduced by light adaptation but enhanced by the intracellular injection of the calcium chelator EGTA. The latter observation indicates that channel activation was not a secondary result of the light-induced rise in intracellular calcium. A twomicroelectrode voltage-clamp method was used to measure the voltage dependence of the light-activated macroscopic conductance. It was found that this conductance is constant over a wide voltage range more negative than zero, but it increases markedly at positive voltages. The single channel currents measured over this same voltage ra