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Authordc.contributor.authorVidal Álvarez, Roberto 
Authordc.contributor.authorMuhsen, Khitam 
Authordc.contributor.authorTennant, Sharon M. 
Authordc.contributor.authorSvennerholm, Ann Mari 
Authordc.contributor.authorSow, Samba O. 
Authordc.contributor.authorSur, Dipika 
Authordc.contributor.authorZaidi, Anita K.M. 
Authordc.contributor.authorFaruque, Abu S.G. 
Authordc.contributor.authorSaha, Debasish 
Authordc.contributor.authorAdegbola, Richard 
Authordc.contributor.authorHossain, M. Jahangir 
Authordc.contributor.authorAlonso, Pedro L. 
Authordc.contributor.authorBreiman, Robert F. 
Authordc.contributor.authorBassat, Quique 
Authordc.contributor.authorTamboura, Boub 
Admission datedc.date.accessioned2019-10-22T03:10:05Z
Available datedc.date.available2019-10-22T03:10:05Z
Publication datedc.date.issued2019
Cita de ítemdc.identifier.citationPLoS Neglected Tropical Diseases, Volumen 13, Issue 1, 2019,
Identifierdc.identifier.issn19352735
Identifierdc.identifier.issn19352727
Identifierdc.identifier.other10.1371/journal.pntd.0007037
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/171859
Abstractdc.description.abstractBackground: Enterotoxigenic Escherichia coli (ETEC) encoding heat-stable enterotoxin (ST) alone or with heat-labile enterotoxin (LT) cause moderate-to-severe diarrhea (MSD) in developing country children. The Global Enteric Multicenter Study (GEMS) identified ETEC encoding ST among the top four enteropathogens. Since the GEMS objective was to provide evidence to guide development and implementation of enteric vaccines and other interventions to diminish diarrheal disease morbidity and mortality, we examined colonization factor (CF) prevalence among ETEC isolates from children age <5 years with MSD and from matched controls in four African and three Asian sites. We also assessed strength of association of specific CFs with MSD. Methodology/Principal findings: MSD cases enrolled at healthcare facilities over three years and matched controls were tested in a standardized manner for many enteropathogens. To identify ETEC, three E. coli colonies per child were tested by polymerase chain reaction (PCR) to detect genes encoding LT, ST; confirmed ETEC were examined by PCR for major CFs (Colonization Factor Antigen I [CFA/I] or Coli Surface [CS] antigens CS1-CS6) and minor CFs (CS7, CS12, CS13, CS14, CS17, CS18, CS19, CS20, CS21, CS30). ETEC from 806 cases had a single toxin/CF profile in three tested strains per child. Major CFs, components of multiple ETEC vaccine candidates, were detected in 66.0% of LT/ST and ST-only cases and were associated with MSD versus matched controls by conditional logistic regression (p≤0.006); major CFs detected in only 25.0% of LT-only cases weren't associated with MSD. ETEC encoding exclusively CS14, identified among 19.9% of 291 ST-only and 1.5% of 259 LT/ST strains, were associated with MSD (p = 0.0011). No other minor CF exhibited prevalence ≥5% and significant association with MSD. Conclusions/Significance: Major CF-based efficacious ETEC vaccines could potentially prevent up to 66% of pediatric MSD cases due to ST-encoding ETEC in developing countries; adding CS14 extends coverage to ~77%.
Lenguagedc.language.isoen
Publisherdc.publisherPublic Library of Science
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourcePLoS Neglected Tropical Diseases
Keywordsdc.subjectPublic Health, Environmental and Occupational Health
Keywordsdc.subjectInfectious Diseases
Títulodc.titleColonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS)
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile