Effects of cryopreservation on mitochondrial function and sperm quality in fish
Author
dc.contributor.author
Figueroa, E.
Author
dc.contributor.author
Lee-Estevez, M.
Author
dc.contributor.author
Valdebenito, I.
Author
dc.contributor.author
Watanabe, I.
Author
dc.contributor.author
Oliveira, R. P.S.
Author
dc.contributor.author
Romero, J.
Author
dc.contributor.author
Castillo, R. L.
Author
dc.contributor.author
Farías, J. G.
Admission date
dc.date.accessioned
2019-10-30T15:22:36Z
Available date
dc.date.available
2019-10-30T15:22:36Z
Publication date
dc.date.issued
2019
Cita de ítem
dc.identifier.citation
Aquaculture, Volumen 511,
Identifier
dc.identifier.issn
00448486
Identifier
dc.identifier.other
10.1016/j.aquaculture.2019.06.004
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/172299
Abstract
dc.description.abstract
The development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In this work, the effects of cryopreservation on ultrastructure, mitochondrial function and antioxidant defences in Atlantic salmon (Salmo salar) spermatozoa were assessed, along with intracellular calcium (Ca2+)i, mitochondrial DNA sequence and sperm function (motility and fertilization rate). Significant ultrastructure alterations of the middle piece and mitochondria were observed in cryopreserved spermatozoa as compared to controls. Oxygen consumption and ATP were also significantly different in cryopreserved spermatozoa, and in spermatozoa incubated with electron transport chain (ETC) uncouplers and inhibitors. Mitochondrial membrane potential, motility, fertilization rate and Ca2+ i in cryopreserved spermatozoa displayed significant reductions compared to fresh spermatozoa. Mitochondrial potential correlated significantly with motility and fertilization rate. A redox imbalance was observed in frozen spermatozoa due to increased lipid peroxidation and superoxide anion production as compared to fresh spermatozoa. Likewise, increased activity of glutathione peroxidase and total glutathione (GSH/GSSG), as well as reduced catalase activity, were observed in comparison with fresh spermatozoa. Our results contribute to a better understanding of cryodamage to mitochondrial functions in fish spermatozoa, and enabled us to establish potential quality assessment indicators. The data suggest that cryopreservation induces a reduction in overall sperm quality and functionality through disruption of the mitochondrial ultrastructure and function, leading to energy depletion and increased oxidative stress. This knowledge may also lead to the identification of a potential biotechnological tool for improving reproductive efficiency in species of commercial and biological interest.