Aquatic thermal reservoirs of microbial life in a remote and extreme high andean hydrothermal system
Author
dc.contributor.author
Pérez, Vilma
Author
dc.contributor.author
Cortés, Johanna
Author
dc.contributor.author
Marchant, Francisca
Author
dc.contributor.author
Dorador, Cristina
Author
dc.contributor.author
Molina, Verónica
Author
dc.contributor.author
Cornejo-D'Ottone, Marcela
Author
dc.contributor.author
Hernández, Klaudia
Author
dc.contributor.author
Jeffrey, Wade
Author
dc.contributor.author
Barahona, Sergio
Author
dc.contributor.author
Hengst, Martha
Admission date
dc.date.accessioned
2020-04-21T23:42:09Z
Available date
dc.date.available
2020-04-21T23:42:09Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Microorganisms 2020, 8, 208
es_ES
Identifier
dc.identifier.other
10.3390/microorganisms8020208
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/173994
Abstract
dc.description.abstract
Hydrothermal systems are ideal to understand how microbial communities cope with challenging conditions. Lirima, our study site, is a polyextreme, high-altitude, hydrothermal ecosystem located in the Chilean Andean highlands. Herein, we analyze the benthic communities of three nearby springs in a gradient of temperature (42-72 degrees C represented by stations P42, P53, and P72) and pH, and we characterize their microbial diversity by using bacteria 16S rRNA (V4) gene metabarcoding and 16S rRNA gene clone libraries (bacteria and archaea). Bacterial clone libraries of P42 and P53 springs showed that the community composition was mainly represented by phototrophic bacteria (Chlorobia, 3%, Cyanobacteria 3%, at P42; Chlorobia 5%, and Chloroflexi 5% at P53), Firmicutes (32% at P42 and 43% at P53) and Gammaproteobacteria (13% at P42 and 29% at P53). Furthermore, bacterial communities that were analyzed by 16S rRNA gene metabarcoding were characterized by an overall predominance of Chloroflexi in springs with lower temperatures (33% at P42), followed by Firmicutes in hotter springs (50% at P72). The archaeal diversity of P42 and P53 were represented by taxa belonging to Crenarchaeota, Diapherotrites, Nanoarchaeota, Hadesarchaeota, Thaumarchaeota, and Euryarchaeota. The microbial diversity of the Lirima hydrothermal system is represented by groups from deep branches of the tree of life, suggesting this ecosystem as a reservoir of primitive life and a key system to study the processes that shaped the evolution of the biosphere.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) - CONICYT FONDECYT: 1100953, 1140356, 1140179, 1181773
CeBiB : FB0001