Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
Author
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Li, Hongjiang
Author
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Wangenheim, Daniel von
Author
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Zhang, Xixi
Author
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Tan, Shutang
Author
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Darwish-Miranda, Nasser
Author
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Naramoto, Satoshi
Author
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Wabnik, Krzysztof
Author
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De Rycke, Riet
Author
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Kaufmann, Walter A.
Author
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Gütl, Daniel
Author
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Tejos, Ricardo
Author
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Grones, Peter
Author
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Ke, Meiyu
Author
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Chen, Xu
Author
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Dettmer, Jan
Author
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Friml, Jiri
Admission date
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2021-01-25T13:17:35Z
Available date
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2021-01-25T13:17:35Z
Publication date
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2020
Cita de ítem
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New Phytologist (2020) 229 (1) 351-369
es_ES
Identifier
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10.1111/nph.16887
Identifier
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https://repositorio.uchile.cl/handle/2250/178303
Abstract
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Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization ofArabidopsisPIN-FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear. Here, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze-fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains. Pharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell-wall components as well as connections between the cell wall and the plasma membrane. This study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems.
es_ES
Patrocinador
dc.description.sponsorship
European Research Council (ERC)
742985
Comision Nacional de Investigacion Cientifica y Tecnologica
CONICYT-PAI 82130047
European Union (EU)
291734