Light-induced release of the cardioprotective peptide angiotensin-(1-9) from thermosensitive liposomes with gold nanoclusters
Author
dc.contributor.author
Bejarano, Julián
Author
dc.contributor.author
Rojas, Aldo
Author
dc.contributor.author
Ramírez Sagredo, Andrea
Author
dc.contributor.author
Riveros Salvatierra, Ana
Author
dc.contributor.author
Morales Zavala, Francisco
Author
dc.contributor.author
Flores, Yvo
Author
dc.contributor.author
Riquelme Meléndez, Jaime
Author
dc.contributor.author
Guzmán, Fanny
Author
dc.contributor.author
Araya, Eyleen
Author
dc.contributor.author
Chiong Lay, Mario
Author
dc.contributor.author
Ocaranza, María Paz
Author
dc.contributor.author
Morales Montecinos, Javier
Author
dc.contributor.author
Villamizar Sarmiento, María Gabriela
Author
dc.contributor.author
Sánchez Vergara, Gina
Author
dc.contributor.author
Lavandero González, Sergio
Author
dc.contributor.author
Kogan, Marcelo J.
Admission date
dc.date.accessioned
2021-06-29T15:08:36Z
Available date
dc.date.available
2021-06-29T15:08:36Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Journal of Controlled Release 328 (2020) 859–872
es_ES
Identifier
dc.identifier.other
10.1016/j.jconrel.2020.11.002
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/180301
Abstract
dc.description.abstract
Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoThermAuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 mu M. This angiotensin-(1-9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43.C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNCbased nanosystem was confirmed using an ex vivo Langendorff heart model.
es_ES
Patrocinador
dc.description.sponsorship
Agencia Nacional de Investigacion y Desarrollo (ANID), Chile
FONDAP 15130011
Puente Pontificia Universidad Catolica de Chile
033/2020
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1190623
1200490
Bayer AG
2017-08-2260
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT PIA/ANILLOS
ACT192144
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
3160323
FONDEQUIP
EQM160157
EQM170111