Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids
Author
dc.contributor.author
Leyton, Allison
Author
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Flores, Liset
Author
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Shene de Vidts, Carolina María
Author
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Chisti, Yusuf
Author
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Larama, Giovanni
Author
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Asenjo de Leuze de Lancizolle, Juan
Author
dc.contributor.author
Armenta, Roberto E.
Admission date
dc.date.accessioned
2021-12-02T14:46:47Z
Available date
dc.date.available
2021-12-02T14:46:47Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
Mar. Drugs 2021, 19, 386.
es_ES
Identifier
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10.3390/md19070386
Identifier
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https://repositorio.uchile.cl/handle/2250/183020
Abstract
dc.description.abstract
Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and carotenoids are needed as
human dietary supplements and are essential components in commercial feeds for the production
of aquacultured seafood. Microorganisms such as thraustochytrids are potential natural sources
of these compounds. This research reports on the lipid and carotenoid production capacity of
thraustochytrids that were isolated from coastal waters of Antarctica. Of the 22 isolates, 21 produced
lipids containing EPA+DHA, and the amount of these fatty acids exceeded 20% of the total fatty
acids in 12 isolates. Ten isolates were shown to produce carotenoids (27.4–63.9 g/g dry biomass).
The isolate RT2316-16, identified as Thraustochytrium sp., was the best producer of biomass (7.2 g/L
in five days) rich in carotenoids (63.9 g/g) and, therefore, became the focus of this investigation.
The main carotenoids in RT2316-16 were -carotene and canthaxanthin. The content of EPA+DHA
in the total lipids (34 3% w/w in dry biomass) depended on the stage of growth of RT2316-16.
Lipid and carotenoid content of the biomass and its concentration could be enhanced by modifying
the composition of the culture medium. The estimated genome size of RT2316-16 was 44 Mb. Of
the 5656 genes predicted from the genome, 4559 were annotated. These included genes of most
of the enzymes in the elongation and desaturation pathway of synthesis of !-3 polyunsaturated
fatty acids. Carotenoid precursors in RT2316-16 were synthesized through the mevalonate pathway.
A -carotene synthase gene, with a different domain organization compared to the gene in other
thraustochytrids, explained the carotenoid profile of RT2316-16.
es_ES
Patrocinador
dc.description.sponsorship
ANID Fondecyt 1200642
Centre for Biotechnology and Bioengineering (CeBiB) FB-0001
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
dc.publisher
MDPI
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States