Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System
Author
dc.contributor.author
Alcamán Arias, María E.
Author
dc.contributor.author
Cifuentes Anticevic, Jerónimo
Author
dc.contributor.author
Diez, Beatriz
Author
dc.contributor.author
Testa, Giovanni
Author
dc.contributor.author
Troncoso, Macarena
Author
dc.contributor.author
Bello, Estrella
Author
dc.contributor.author
Farías, Laura
Admission date
dc.date.accessioned
2022-06-29T21:26:31Z
Available date
dc.date.available
2022-06-29T21:26:31Z
Publication date
dc.date.issued
2022
Cita de ítem
dc.identifier.citation
Frontiers in Microbiology March 2022 Volume 13 Article Number 821902
es_ES
Identifier
dc.identifier.other
10.3389/fmicb.2022.821902
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/186351
Abstract
dc.description.abstract
Marine ammonia oxidizers that oxidize ammonium to nitrite are abundant in polar waters,
especially during the winter in the deeper mixed-layer of West Antarctic Peninsula (WAP)
waters. However, the activity and abundance of ammonia-oxidizers during the summer
in surface coastal Antarctic waters remain unclear. In this study, the ammonia-oxidation
rates, abundance and identity of ammonia-oxidizing bacteria (AOB) and archaea (AOA)
were evaluated in the marine surface layer (to 30m depth) in Chile Bay (Greenwich Island,
WAP) over three consecutive late-summer periods (2017, 2018, and 2019). Ammoniaoxidation rates of 68.31nmolNL−1
day−1
(2018) and 37.28nmolNL−1
day−1
(2019) were
detected from illuminated 2m seawater incubations. However, high ammonia-oxidation
rates between 267.75 and 109.38nmolNL−1
day−1
were obtained under the dark condition
at 30m in 2018 and 2019, respectively. During the late-summer sampling periods both
stratifying and mixing events occurring in the water column over short timescales
(February–March). Metagenomic analysis of seven nitrogen cycle modules revealed the
presence of ammonia-oxidizers, such as the Archaea Nitrosopumilus and the Bacteria
Nitrosomonas and Nitrosospira, with AOA often being more abundant than AOB. However,
quantification of specific amoA gene transcripts showed number of AOB being two orders
of magnitude higher than AOA, with Nitrosomonas representing the most transcriptionally
active AOB in the surface waters. Additionally, Candidatus Nitrosopelagicus and
Nitrosopumilus, phylogenetically related to surface members of the NP-ε and NP-γ clades
respectively, were the predominant AOA. Our findings expand the known distribution of
ammonium-oxidizers to the marine surface layer, exposing their potential ecological role
in supporting the marine Antarctic system during the productive summer periods.
es_ES
Patrocinador
dc.description.sponsorship
ANID/PFCHA/Doctorado Nacional 2017 21170561
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
dc.publisher
Frontiers Media
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States