Browsing by Author "Bezanilla, Francisco"

Now showing items 1-7 of 7

    • A conducting state with properties of a slow inactivated state in a Shaker K+ channel mutant 
      Olcese, Riccardo; Sigg, Daniel; Latorre, Ramón; Bezanilla, Francisco; Stefani, Enrico (ROCKEFELLER UNIV PRESS, 2001-02)
      In Shaker K+ channel, the amino terminus deletion Delta6-46 removes fast inactivation (N-type) unmasking a slow inactivation process. In Shaker Delta6-46 (Sh-IR) background, two additional mutations (T449V-I470C) remove ...
    • Correlation between charge movement and ionic current during slow inactivation in Shaker K+ channels 
      Olcese, Riccardo; Latorre, Ramón; Toro, Ligia; Bezanilla, Francisco; Stefani, Enrico (ROCKEFELLER UNIV PRESS, 1997-11)
      Prolonged depolarization induces a slow inactivation process in some Ki channels. We have studied ionic and gating currents during long depolarizations in the mutant Shaker H4-Delta(6-46) K+ channel and in the nonconducting ...
    • Deletion of the S3-S4 linker in the Shaker potassium channel reveals two quenching groups near the outside of S4 
      Sorensen, J. B.; Cha, A.; Latorre, Ramón; Rosenman, Eduardo; Bezanilla, Francisco (ROCKEFELLER UNIV PRESS, 2000-02)
      When attached outside the voltage-sensing S4 segment of the Shaker potassium channel, the fluorescent probe tetramethylrhodamine (TMRM) undergoes voltage-dependent fluorescence changes (Delta F) due to differential interaction ...
    • Determination of the stoichiometry between alpha- and gamma 1 subunits of the BK channel using LRET 
      Carrasquel Ursulaez, Willy; Alvarez, Osvaldo; Bezanilla, Francisco; Latorre, Ramón (Cell Press, 2018)
      Two families of accessory proteins, beta and gamma, modulate BK channel gating and pharmacology. Notably, in the absence of internal Ca2+, the gamma 1 subunit promotes a large shift of the BK conductance-voltage curve to ...
    • Mechanism of potassium ion uptake by the Na+/K+-ATPase 
      Castillo, Juan P.; Rui, Huan; Basilio Seyler, Daniel; Das, Avisek; Roux, Benoit; Latorre, Ramón; Bezanilla, Francisco; Holmgren, Miguel (Nature, 2015)
      The Na+/ K+-ATPase restores sodium (Na+) and potassium (K+) electrochemical gradients dissipated by action potentials and ion-coupled transport processes. As ions are transported, they become transiently trapped between ...
    • Modulation of the Shaker K+ channel gating kinetics by the S3-S4 linker 
      González, Carlos; Rosenman, Eduardo; Bezanilla, Francisco; Álvarez Araya, Osvaldo; Latorre, Ramón (ROCKEFELLER UNIV PRESS, 2000-02)
      In Shaker K+ channels depolarization displaces outwardly the positively charged residues of the S4 segment. The amount of this displacement is unknown, but large movements of the S4 segment should be con strained by the ...
    • Periodic perturbations in Shaker K+ channel gating kinetics by deletions in the S3-S4 linker 
      González, Carlos; Rosenman, Eduardo; Bezanilla, Francisco; Álvarez Araya, Osvaldo; Latorre, Ramón (NATL ACAD SCIENCES, 2001-08-14)
      Upon depolarization positive charges contained in the transmembrane segment S4 of voltage-dependent channels are displaced from the cytoplasmic to the external milieu, This charge movement leads to channel opening. In ...