Browsing by Author "Espinosa, Alejandra"

Now showing items 1-18 of 18

    • Assessment of red blood cell glutathione status in insulin resistance 
      Galgani Fuentes, José; Vásquez, Karla; Leonelli, Giannella; Espinosa, Alejandra; Araya, Hector; Pérez Bravo, Francisco (2012)
      The aim of this study was to assess red blood cell glutathione from insulin-sensitive and insulin-resistant individuals before and after an oral glucose dose. Fifteen healthy, young (24 ± 5 years), nonobese (23 ± 2 kg·m ...
    • Capacitative calcium entry in testosterone-induced intracellular calcium oscillations in myotubes 
      Estrada Hormazábal, Manuel; Espinosa, Alejandra; Gibson, C. J.; Uhlen, P.; Jaimovich Pérez, Enrique (SOC ENDOCRINOLOGY, 2005-02)
      Ca2+ oscillations are one of the most important signals within the cell. The mechanism for generation of Ca2+ oscillations is still not yet fully elucidated. We studied the role of capacitative Ca2+ entry (CCE) on intracellular ...
    • Effects of rosa mosqueta oil supplementation in lipogenic markers associated with prevention of liver steatosis 
      Dossi, Camila G.; Cadagan, Cynthia; San Martín, Marcela; Espinosa, Alejandra; González Mañán, Daniel Alonso; Silva, David; Mancilla, Rodrigo A.; Tapia, Gladys S. (Royal Society of Chemistry, 2017)
      © The Royal Society of Chemistry. Rosa mosqueta (RM) oil is rich in α-linolenic acid (ALA)-a precursor of eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), and it has a high antioxidant activity due to its abundant ...
    • Electrical stimuli release ATP to increase GLUT4 translocation and glucose uptake via PI3Kγ-Akt-AS160 in ... 
      Osorio Fuentealba, César; Contreras Ferrat, Ariel Eduardo; Altamirano, Francisco; Espinosa, Alejandra; Li, Qing; Niu, Wenyan; Lavandero González, Sergio; Klip, Amira; Jaimovich Pérez, Enrique (2013)
      Skeletal muscle glucose uptake in response to exercise is preserved in insulin-resistant conditions, but the signals involved are debated. ATP is released from skeletal muscle by contractile activity and can autocrinely ...
    • IGF-I and insulin induce different intracellular calcium signals in skeletal muscle cells 
      Espinosa, Alejandra; Estrada Hormazábal, Manuel; Jaimovich Pérez, Enrique (SOC ENDOCRINOLOGY, 2004-08)
      We studied the effect of IGF-I and insulin on intracellular Ca2+ in primary cultured myotubes. IGF-I induced a fast and transient Ca2+ increase, measured as fluo-3 fluorescence. This response was blocked by both genistein ...
    • Insulin elicits a ROS-activated and an IP3-dependent Ca2+ release, which both impinge on GLUT4 translocation 
      Contreras Ferrat, Ariel Eduardo; Llanos Vidal, Paola; Vásquez, César; Espinosa, Alejandra; Osorio Fuentealba, César; Arias Calderón, Manuel; Lavandero González, Sergio; Klip, Amira; Hidalgo Tapia, María Cecilia; Jaimovich Pérez, Enrique (The Company of Biologists Ltd, 2014)
      Insulin signaling includes generation of low levels of H2O2; however, its origin and contribution to insulin-stimulated glucose transport are unknown. We tested the impact of H2O2 on insulin-dependent glucose transport ...
    • Insulin-Dependent H2O2 Production Is Higher in Muscle Fibers of Mice Fed with a High-Fat Diet 
      Espinosa, Alejandra; Campos, Cristian; Díaz Vegas, Alexis; Galgani Fuentes, José; Juretic Díaz, Nevenka Militza; Osorio Fuentealba, César; Bucarey, José L.; Tapia, Gladys; Valenzuela, Rodrigo; Contreras Ferrat, Ariel Eduardo; Llanos Vidal, Paola; Jaimovich Pérez, Enrique (2013)
      Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with a high-fat diet (HFD) are a model of insulin resistance. In skeletal muscle, hydrogen peroxide (H2O2) ...
    • Molecular adaptations underlying the beneficial effects of hydroxytyrosol in the pathogenic alterations ... 
      Valenzuela Báez, Rodrigo; Illesca, Paola; Echeverría, Francisca; Espinosa, Alejandra; Rincón Cervera, Miguel Ángel; Ortíz, Macarena; Hernández Rodas, María Catalina; Valenzuela Bonomo, Carlos; Videla Cabrera, Luis (Royal Society of Chemistry, 2017)
      © 2017 The Royal Society of Chemistry.Scope: Non-alcoholic fatty liver disease (NAFLD) is a condition characterized by an increment in the liver fat content, with a concomitant reduction in the content of n-3-long chain ...
    • Molecular mechanisms related to the hepatoprotective effects of antioxidant-rich extra virgin olive oil ... 
      Barrera, Cynthia; Valenzuela, Rodrigo; Rincón, Miguel Ángel; Espinosa, Alejandra; Echeverria, Francisca; Romero, Nalda; Gonzalez-Mañan, Daniel; Videla Cabrera, Luis (Elsevier Inc., 2018)
      © 2018 Elsevier Inc. Enhanced iron levels in liver are associated with oxidative stress development and damage with increased fat accumulation. The aim of this work was to assess the hypothesis that antioxidant-rich extra ...
    • Myotube depolarization generates reactive oxygen species through NAD(P)H oxidase; ROS-elicited Ca2+ stimulates ... 
      Espinosa, Alejandra; Leiva, Aída; Peña, Marisol; Müller, Mariolly; Debandi Cuadra, Aníbal; Hidalgo Tapia, María Cecilia; Carrasco Friz, María Angélica; Jaimovich Pérez, Enrique (WILEY-LISS, DIV JOHN WILEY & SONS INC, 2006-11)
      Controlled generation of reactive oxygen species (ROS) may contribute to physiological intracellular signaling events. We determined ROS generation in primary cultures of rat skeletal muscle after field stimulation (4001-ms ...
    • N-3 Long-Chain Polyunsaturated Fatty Acid Supplementation Significantly Reduces Liver Oxidative Stress in ... 
      Valenzuela, Rodrigo; Espinosa, Alejandra; González Mañán, Daniel Alonso; D'Espessailles Tapia, Amanda; Fernández Arancibia, Virginia; Videla Cabrera, Luis; Tapia Opazo, Gladys (2012)
      Omega-3 (n-3) long-chain polyunsaturated fatty acids (n-3 LCPUFA) are associated with several physiological functions, suggesting that their administration may prevent non transmissible chronic diseases. Therefore, we ...
    • N-3 long-chain PUFA supplementation prevents high fat diet induced mouse liver steatosis and inflammation ... 
      Tapia Opazo, Gladys; Valenzuela, Rodrigo; Espinosa, Alejandra; Romanque, Pamela; Dossi, Camila; González Mañán, Daniel Alonso; Videla Cabrera, Luis; D'Espessailles Tapia, Amanda (Wiley-VCH Verlag, 2014)
      Scope: Dietary n-3 long-chain PUFAs (n-3 LCPUFAs) supplementation was studied in an HFD-induced (HFD is high-fat diet) steatosis and inflammation in relation to peroxisome proliferator-activated receptor alpha (PPAR-α) and ...
    • NADPH oxidase and hydrogen peroxide mediate insulin-induced calcium increase in skeletal muscle cells 
      Espinosa, Alejandra; García, Alejandra; Härtel, Steffen; Hidalgo Tapia, María Cecilia; Jaimovich Pérez, Enrique (2009)
      Skeletal muscle is one of the main physiological targets of insulin, a hormone that triggers a complex signaling cascade and that enhances the production of reactive oxygen species (ROS) in different cell types. ROS, ...
    • Possible link of different slow calcium signals generated by membrane potential and hormones to differential ... 
      Jaimovich Pérez, Enrique; Espinosa, Alejandra (SOCIEDAD BIOLOGIA CHILE, 2004)
      The study of fluorescent calcium signals from cultured rat myotubes has provided interesting results in the past few years. Both K+ depolarization and tetanic electrical stimulation were shown to produce slow Ca2+ signals, ...
    • Reduction in the desaturation capacity of the liver in mice subjected to high fat diet: Relation to LCPUFA ... 
      Valenzuela Báez, Rodrigo; Barrera Ramírez, Cynthia; Espinosa, Alejandra; Llanos Vidal, Paola; Orellana, Paula; Videla Cabrera, Luis (Elsevier, 2015)
      alpha-Linolenic (ALA) and linoleic (LA) acids are precursors of long chain polyunsaturated fatty acids (LCPUFAs), FAs with important biochemical and physiological functions. In this process, desaturation reactions catalyzed ...
    • ROS production via P2Y1-PKC-NOX2 is triggered by extracellular ATP after electrical stimulation of skeletal ... 
      Díaz Vegas, Alexis; Campos, Cristian A.; Contreras Ferrat, Ariel Eduardo; Casas, Mariana; Buvinic, Sonja; Jaimovich Pérez, Enrique; Espinosa, Alejandra (Public Library of Science, 2015)
      © 2015 Díaz-Vegas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the ...
    • The cholesterol-lowering agent methyl-β-cyclodextrin promotes glucose uptake via GLUT4 in adult muscle ... 
      Llanos Vidal, Paola; Contreras Ferrat, Ariel; Georgiev, Tihomir; Osorio Fuentealba, César; Espinosa, Alejandra; Hidalgo, Jorge; Hidalgo Tapia, María Cecilia; Jaimovich Pérez, Enrique (American Physiological Society, 2015)
      Insulin stimulates glucose uptake in adult skeletal muscle by promoting the translocation of GLUT4 glucose transporters to the transverse tubule (T-tubule) membranes, which have particularly high cholesterol levels. We ...
    • Vegetable oils rich in alpha linolenic acid incremen the paticn-3 LCPUFA, modulating the fatty acid metabolism ... 
      Rincón Cervera, Miguel Ángel; Valenzuela Báez, Rodrigo; Hernández Rodas, María Catalina; Barrera Ramírez, Cynthia; Espinosa, Alejandra; Marambio, Macarena; Valenzuela Bonomo, Carlos (2016)
      Alpha-linolenic acid (C18:3 n-3, ALA) is an essential fatty acid and the metabolic precursor of long-chain polyunsaturated fatty acids (LCPUFA) from the n-3 family with relevant physiological and metabolic roles: ...